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A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration
Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use w...
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| Published in: | Analytical biochemistry 2015-10, Vol.486, p.78-80 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c350t-6070775c2c3d3dd370031e889f01dfcb9d7ee639c0d8576a66ecb6ada35075313 |
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| cites | cdi_FETCH-LOGICAL-c350t-6070775c2c3d3dd370031e889f01dfcb9d7ee639c0d8576a66ecb6ada35075313 |
| container_end_page | 80 |
| container_issue | |
| container_start_page | 78 |
| container_title | Analytical biochemistry |
| container_volume | 486 |
| creator | Rupprecht, Kevin R. Lang, Ewa Z. Gregory, Svetoslava D. Bergsma, Janet M. Rae, Tracey D. Fishpaugh, Jeffrey R. |
| description | Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use with protein solutions where SDS concentration is a critical factor, so we modified this method after examining multiple parameters (solvent, pH, buffers, and light exposure). The improved method is simple to implement, robust, accurate, and (most important) precise. |
| doi_str_mv | 10.1016/j.ab.2015.06.013 |
| format | article |
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The improved method is simple to implement, robust, accurate, and (most important) precise.</description><subject>Concentration</subject><subject>Hydrogen-Ion Concentration</subject><subject>Light</subject><subject>Limit of Detection</subject><subject>Quantitative analysis</subject><subject>Reproducibility of Results</subject><subject>Sodium dodecyl sulfate (SDS)</subject><subject>Sodium Dodecyl Sulfate - analysis</subject><subject>Solvents - chemistry</subject><subject>Spectrophotometry - methods</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PwzAQhi0EoqWwM6GMLAnnurEbtgrxJSGxAKvl2BfqKomD7SD132NoYWO6G5731d1DyDmFggLlV5tC1cUcaFkAL4CyAzKlUPEcGFSHZAoALJ_zSkzISQgbAEoXJT8mkzmnJUC1mJK3VTZ41DZgFgbU0bth7aLrMHqrszTWzmSN82lVYfS2f8-CM3bsMuMM6m2bhbFtVMRMu15jH72K1vWn5KhRbcCz_ZyR17vbl5uH_On5_vFm9ZRrVkLMOQgQotRzzQwzhol0MMXlsmqAmkbXlRGInFUazLIUXHGOuubKqJQWJaNsRi53vYN3HyOGKDsbNLat6tGNQVIBC7ZYwg8KO1R7F4LHRg7edspvJQX5bVNupKrlt00JXCabKXKxbx_rDs1f4FdfAq53AKYfPy16GbTF5MHYJDVK4-z_7V-REoUT</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Rupprecht, Kevin R.</creator><creator>Lang, Ewa Z.</creator><creator>Gregory, Svetoslava D.</creator><creator>Bergsma, Janet M.</creator><creator>Rae, Tracey D.</creator><creator>Fishpaugh, Jeffrey R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151001</creationdate><title>A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration</title><author>Rupprecht, Kevin R. ; Lang, Ewa Z. ; Gregory, Svetoslava D. ; Bergsma, Janet M. ; Rae, Tracey D. ; Fishpaugh, Jeffrey R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-6070775c2c3d3dd370031e889f01dfcb9d7ee639c0d8576a66ecb6ada35075313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Concentration</topic><topic>Hydrogen-Ion Concentration</topic><topic>Light</topic><topic>Limit of Detection</topic><topic>Quantitative analysis</topic><topic>Reproducibility of Results</topic><topic>Sodium dodecyl sulfate (SDS)</topic><topic>Sodium Dodecyl Sulfate - analysis</topic><topic>Solvents - chemistry</topic><topic>Spectrophotometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rupprecht, Kevin R.</creatorcontrib><creatorcontrib>Lang, Ewa Z.</creatorcontrib><creatorcontrib>Gregory, Svetoslava D.</creatorcontrib><creatorcontrib>Bergsma, Janet M.</creatorcontrib><creatorcontrib>Rae, Tracey D.</creatorcontrib><creatorcontrib>Fishpaugh, Jeffrey R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rupprecht, Kevin R.</au><au>Lang, Ewa Z.</au><au>Gregory, Svetoslava D.</au><au>Bergsma, Janet M.</au><au>Rae, Tracey D.</au><au>Fishpaugh, Jeffrey R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>486</volume><spage>78</spage><epage>80</epage><pages>78-80</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. 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| ispartof | Analytical biochemistry, 2015-10, Vol.486, p.78-80 |
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| language | eng |
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| source | Elsevier |
| subjects | Concentration Hydrogen-Ion Concentration Light Limit of Detection Quantitative analysis Reproducibility of Results Sodium dodecyl sulfate (SDS) Sodium Dodecyl Sulfate - analysis Solvents - chemistry Spectrophotometry - methods |
| title | A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration |
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