Lophozozymus pictor toxin: A fluorescent structural isomer of palytoxin

Purified Lophozozymus pictor toxin (LPTX) shares many properties similar to palytoxin (PTX). LPTX and palytoxin isolated from Palythoa caribaeorum (C-PTX) have similar mol. wts of approx. 2680 on ionspray mass spectrometry (MS). In addition, antibodies against PTX could recognize and bind LPTX. Mixe...

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Bibliographic Details
Published in:Toxicon (Oxford) 1995-10, Vol.33 (10), p.1373-1377
Main Authors: Lau, C.O, Tan, C.H, Khoo, H.E, Yuen, R, Lewis, R.J, Corpuz, G.P, Bignami, G.S
Format: Article
Language:English
Subjects:
Acrylamides - analysis
Acrylamides - chemistry
Animal poisons toxicology. Antivenoms
Antibodies, Monoclonal
Biological and medical sciences
Chromatography, High Pressure Liquid
Cnidarian Venoms - analysis
Cnidarian Venoms - chemistry
Enzyme-Linked Immunosorbent Assay
Fluorescence
Lophozozymus pictor
Marine
Medical sciences
Palythoa caribaeorum
Spectrophotometry, Ultraviolet
Stereoisomerism
Terpenes - analysis
Terpenes - chemistry
Toxicology
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Summary:Purified Lophozozymus pictor toxin (LPTX) shares many properties similar to palytoxin (PTX). LPTX and palytoxin isolated from Palythoa caribaeorum (C-PTX) have similar mol. wts of approx. 2680 on ionspray mass spectrometry (MS). In addition, antibodies against PTX could recognize and bind LPTX. Mixed mode high-performance liquid chromatography (HPLC) of LPTX, C-PTX and H-PTX (isolated from Palythoa tuberculosa) showed a major PTX component common to all three with the characteristic PTX-like UV spectrum at a retention time ( R t) of 17 min. However, LPTX exhibits fluorescence but PTX of equivalent toxicity does not. LPTX showed a unique peak at R t of approx. 22 min on mixed mode HPLC. In addition, LPTX and C-PTX showed different ion fragmentation patterns on MS/MS. These results suggest that LPTX and the palytoxins are structural isomers, containing at least one difference which gives rise to fluorescence in LPTX.
ISSN:0041-0101
1879-3150
DOI:10.1016/0041-0101(95)00075-W