Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of live propagules of Trichoderma harzianum in a peat-bran medium

Mouse monoclonal antibodies (MAbs) and mouse and rabbit (polyclonal) antisera were raised against a Trichoderma harzianum isolate T95. A cell line, Th.HD3 3, raised from splenocytes from a mouse immunized with lyophilized mycelium and Quil A adjuvant, produced MAbs of the immunoglobulin sub-class Ig...

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Bibliographic Details
Published in:Soil biology & biochemistry 1994, Vol.26 (7), p.909-920
Main Authors: Thornton, C.R., Dewey, F.M., Gilugan, C.A.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biological control
Control
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Phytopathology. Animal pests. Plant and forest protection
Trichoderma harzianum
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Summary:Mouse monoclonal antibodies (MAbs) and mouse and rabbit (polyclonal) antisera were raised against a Trichoderma harzianum isolate T95. A cell line, Th.HD3 3, raised from splenocytes from a mouse immunized with lyophilized mycelium and Quil A adjuvant, produced MAbs of the immunoglobulin sub-class IgG 2a that were specific recognizing, by ELISA, antigens from a number of Trichoderma species and species from the related genus Hypocrea but not from a range of other soil-borne fungi. These MAbs were used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of live propagules of Trichoderma harzianum in a peat-bran medium. Heat, protease and periodate treatment of the T95 antigen and binding on Western blots indicate that the Th.HD3 3 MAbs recognized a protein epitope of ca M r 18,500 which forms part of a larger glycoprotein molecule.
ISSN:0038-0717
1879-3428
DOI:10.1016/0038-0717(94)90307-7