Effect of gelation temperature and gel-dissolving solution on cell viability and recovery of two Pseudomonas putida strains co-immobilized within calcium alginate or Kappa -carrageenan gel beads

Since a lethal effect of an increased temperature (42 degree C) on Pseudomonas putida strains PaW8 and PaW130 was demonstrated, strictly ionotropic gels such as calcium alginate or Kappa -carrageenan type X 0909 were used for cell co-immobilization, rather than a thermoionotropic Kappa -carrageenan...

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Bibliographic Details
Published in:Biotechnology techniques 1995-10, Vol.9 (10), p.747-752
Main Authors: Mater, DDG, Barbotin, J-N, Nava Saucedo, JE, Truffaut, N, Thomas, D
Format: Article
Language:English
Subjects:
Pseudomonas putida
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Summary:Since a lethal effect of an increased temperature (42 degree C) on Pseudomonas putida strains PaW8 and PaW130 was demonstrated, strictly ionotropic gels such as calcium alginate or Kappa -carrageenan type X 0909 were used for cell co-immobilization, rather than a thermoionotropic Kappa -carrageenan gel. Among the variety of gel-dissolving solutions tested, only a 0.05M Na sub(2)CO sub(3)/0.02M citric acid solution was able to preserve around 100 % of the cell viability. A complete cell recovery was obtained from calcium alginate gel beads, while only 6 % of viable cells was recovered from the ionotropic Kappa -carrageenan gel.
ISSN:0951-208X
DOI:10.1007/BF00159242