Communication: Antibody stability and behavior on surfaces

Antibody microarrays have the potential to revolutionize molecular detection in scientific, medical, and other biosensor applications, but their current use is limited because of poor reliability. It is hypothesized that one reason for their poor performance results from strong antibody-surface inte...

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Bibliographic Details
Published in:The Journal of chemical physics 2015-08, Vol.143 (6), p.061101-061101
Main Authors: Bush, Derek B, Knotts, 4th, Thomas A
Format: Article
Language:English
Subjects:
Antibodies
Antibodies - chemistry
Biosensors
Coarsening
Hydrophobic and Hydrophilic Interactions
Immunoglobulins
Models, Chemical
Physics
Proteins
Solutions - chemistry
Surface Properties
Surface stability
Temperature
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Summary:Antibody microarrays have the potential to revolutionize molecular detection in scientific, medical, and other biosensor applications, but their current use is limited because of poor reliability. It is hypothesized that one reason for their poor performance results from strong antibody-surface interactions that destabilize the antibody structure and create steric interference for antigen recognition. Using a recently developed coarse-grain protein-surface model that has been parameterized against experimental data, antibody-surface interactions for two antibody orientations on two types of surfaces have been investigated. The results show that regardless of attachment geometry, antibodies tend to collapse onto hydrophobic surfaces and exhibit lower overall stability compared to antibodies on hydrophilic surfaces or in bulk solution. The results provide an unprecedented view into the dynamics of antibodies on surfaces and offer new insights into the poor performance exhibited by current antibody microarrays.
ISSN:0021-9606
1089-7690
DOI:10.1063/1.4928455