Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8

[Display omitted] l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2015-09, Vol.25 (18), p.3980-3983
Main Authors: Li, Zijie, Wu, Xiaoru, Cai, Li, Duan, Shenglin, Liu, Jia, Yuan, Peng, Nakanishi, Hideki, Gao, Xiao-Dong
Format: Article
Language:English
Subjects:
Aldehyde-Lyases - metabolism
Aldolase
Fructose - biosynthesis
Fructose - chemistry
Hexoses - biosynthesis
Hexoses - chemistry
Molecular Structure
Rare sugar
RhaD
Sorbose - biosynthesis
Sorbose - chemistry
Synthesis
Thermotoga maritima
Thermotoga maritima - enzymology
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Summary:[Display omitted] l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars d-psicose, d-sorbose, l-tagatose and l-fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2015.07.027