Labedella endophytica sp. nov., a novel endophytic actinobacterium isolated from stem of Anabasis elatior (C. A. Mey.) Schischk

• Published in: Antonie van Leeuwenhoek 2015-01, Vol.107 (1), p.95-102
• Main Authors: Wang, Hong-Fei, Zhang, Yong-Guang, Cheng, Juan, Hozzein, Wael N, Liu, Wei-Hong, Li, Li, Chen, Ji-Yue, Guo, Jian-Wei, Zhang, Yuan-Ming, Li, Wen-Jun
• Format: Article
• Language: English
• Subjects: Actinobacteria - classification; Actinobacteria - isolation & purification; Amaranthaceae - microbiology; Anabasis; Bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Catalase - metabolism; Cell Wall - chemistry; chemotaxonomy; China; Cluster Analysis; Cytosol - chemistry; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; endophytes; Endophytes - classification; Endophytes - isolation & purification; fatty acids; Fatty Acids - analysis; halophytes; Life Sciences; Medical Microbiology; menaquinones; Microbiology; Microscopy, Electron, Scanning; Molecular Sequence Data; new species; nucleotide sequences; Original Paper; ornithine; Ornithine - analysis; Oxidoreductases - metabolism; Phylogeny; Pigments, Biological - metabolism; Plant Sciences; Plant Stems - microbiology; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; Soil Science & Conservation; Vitamin K 2 - analysis
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0307-0

A Gram-stain positive, yellow-coloured, aerobic, non-motile, catalase-positive and oxidase-negative, endophytic actinobacterium, designated strain EGI 6500705ᵀ, was isolated from the surface-sterilized stem of a halophyte Anabasis elatior (C. A. Mey.) Schischk collected from Urumqi, Xinjiang province, north-west China. The organism had ornithine as the diagnostic cell-wall diamino acid. The major fatty acids were anteiso-C₁₅:₀, anteiso-C₁₇:₀and iso-C₁₆:₀. The predominant menaquinones were MK-9, MK-10 and MK-11. The DNA G + C content of strain EGI 6500705ᵀwas 69.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain EGI 6500705ᵀis clearly affiliated with the genus Labedella and most closely related to Labedella gwakjiensis KCTC 19176ᵀ, with 99.0 % sequence similarity. DNA–DNA relatedness between strain EGI 6500705ᵀand L. gwakjiensis KCTC 19176ᵀwas 27.4 %. On the basis of phenophytic, chemotaxonomic and phylogenetic analysis, the strain EGI 6500705ᵀrepresents a novel species of the genus Labedella, for which the name Labedella endophytica sp. nov. is proposed. The type strain is EGI 6500705ᵀ(=KCTC 29494ᵀ = CPCC 203961ᵀ = JCM 30092ᵀ).