Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China

• Published in: Antonie van Leeuwenhoek 2015-01, Vol.107 (1), p.1-7
• Main Authors: Zhao, Shuai, Li, Li, Li, Shan-Hui, Wang, Hong-Fei, Hozzein, Wael N, Zhang, Yong-Guang, Wadaan, Mohammed A. M, Li, Wen-Jun, Tian, Chang-Yan
• Format: Article
• Language: English
• Subjects: Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Actinobacteria - physiology; Aerobiosis; Bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Carbohydrates - analysis; Cell Wall - chemistry; chemotaxonomy; Chenopodiaceae - microbiology; China; Cluster Analysis; Cytosol - chemistry; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; fatty acids; Fatty Acids - analysis; glucose; halophytes; Life Sciences; Lipids; mannose; Medical Microbiology; Microbiology; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Molecular Sequence Data; new species; nucleotide sequences; Original Paper; Peptidoglycan - analysis; peptidoglycans; phospholipids; Phospholipids - analysis; Phylogeny; Physophora; Plant Sciences; Polymorphism; Quinones - analysis; rhamnose; ribose; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Salt-Tolerant Plants - microbiology; Sequence Analysis, DNA; sequence homology; Soil Science & Conservation; Suaeda
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0297-y

A Gram-stain-positive, aerobic, non-motile, coryneform bacterium, designated strain EGI 60002ᵀ, was isolated from the halophyte Suaeda physophora. Cells were coryneform shaped and polymorphic. Phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate was closely related to Actinotalea ferrariae CF5-4ᵀ(95.8 % gene sequence similarity). The peptidoglycan type of strain EGI 60002ᵀwas A4β, containing L-Orn-D-Ser-D-Asp. The cell-wall sugars were mannose, ribose, rhamnose and glucose. The major fatty acids (>5 %) of strain EGI 60002ᵀwere iso-C₁₄:₀, iso-C₁₅:₀, anteiso-C₁₅:₁A and anteiso-C₁₅:₀. The predominant respiratory quinone was MK-10(H₄). The major polar lipids were diphosphatidylglycerol (DPG), one unidentified phosphoglycolipid (PGL) and one unidentified phospholipid (PL1). The genomic DNA G+C content was 72.3 mol%. On the basis of morphological, physiological, chemotaxonomic data, and phylogenetic analysis, strain EGI 60002ᵀshould be classified as a novel species within the genus Actinotalea, for which the name Actinotalea suaedae sp. nov. is proposed. The type strain is EGI 60002T (=JCM 19624ᵀ = KACC 17839ᵀ = KCTC 29256ᵀ).