Field‐evolved resistance to four modes of action of herbicides in a single kochia (Kochia scoparia L. Schrad.) population

• Published in: Pest management science 2015-09, Vol.71 (9), p.1207-1212
• Main Authors: Varanasi, Vijay K, Godar, Amar S, Currie, Randall S, Dille, Anita J, Thompson, Curtis R, Stahlman, Phillip W, Jugulam, Mithila
• Format: Article
• Language: English
• Subjects: 3-phosphoshikimate 1-carboxyvinyltransferase; acetolactate synthase; ALS gene; Atrazine; auxins; Bassia scoparia; Bassia scoparia - genetics; Bassia scoparia - physiology; broadleaf weeds; chlorsulfuron; Crop fields; Crop production; Dicamba; DNA, Plant - genetics; Economic importance; enzyme inhibitors; EPSPS gene; evolution; Evolutionary biology; genes; Genetics; Glycine - analogs & derivatives; Glyphosate; Herbicide Resistance; Herbicides; Herbs; Kansas; Kochia; Kochia scoparia; mechanism of action; mechanism of resistance; multiple herbicide resistance; Mutation; photosystem II; Plant resistance; Plant Weeds; psbA gene; resistance mechanisms; Sulfonamides; Survival analysis; Triazines; Weed control; Weeds
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.4034

BACKGROUND: Evolution of multiple herbicide resistance in weeds is a serious threat to weed management in crop production. Kochia is an economically important broadleaf weed in the US Great Plains. This study aimed to confirm resistance to four sites of action of herbicides in a single kochia (Kochia scoparia L. Schrad.) population from a crop field near Garden City (GC), Kansas, and further determine the underlying mechanisms of resistance. RESULTS: One‐fourth of the GC plants survived the labeled rate or higher of atrazine [photosystem II (PSII) inhibitor], and the surviving plants had the Ser‐264 to Gly mutation in the psbA gene, the target site of atrazine. Results showed that 90% of GC plants survived the labeled rate of dicamba, a synthetic auxin. At least 87% of the plants survived up to 72 g a.i. ha⁻¹ of chlorsulfuron [acetolactate synthase (ALS) inhibitor], and analysis of the ALS gene revealed the presence of Pro‐197 to Thr and/or Trp‐574 to Lue mutation(s). Most GC plants also survived the labeled rate of glyphosate [5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS) inhibitor), and the resistant plants had 5–9 EPSPS gene copies (relative to the ALS gene). CONCLUSION: We confirm the first case of evolution of resistance to four herbicide sites of action (PSII, ALS and EPSPS inhibitors and synthetic auxins) in a single kochia population, and target‐site‐based mechanisms confer resistance to atrazine, glyphosate and chlorsulfuron. © 2015 Society of Chemical Industry