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Nineteen polymorphic microsatellite markers developed for Trachinotus ovatus

To evaluate the population genetic diversity of the ovate pompano, we isolated and characterized 19 microsatellite markers using a (CA)13-enriched genomic library. Polymorphism was assessed in 30 individuals from a single population collected from the Daya Bay Aquaculture Center, Guangdong, China. T...

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Bibliographic Details
Published in:Genetics and molecular research 2014-12, Vol.13 (4), p.10518-10522
Main Authors: Xie, Z Z, Huang, M W, Xu, W, Peng, C, He, J N, Meng, Z N, Zhang, Y, Li, S S, Lin, H R
Format: Article
Language:English
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Summary:To evaluate the population genetic diversity of the ovate pompano, we isolated and characterized 19 microsatellite markers using a (CA)13-enriched genomic library. Polymorphism was assessed in 30 individuals from a single population collected from the Daya Bay Aquaculture Center, Guangdong, China. The number of alleles per locus ranged from 2 to 18 with an average of 7.8. The observed and expected heterozygosities varied from 0.2667 to 1.000 and from 0.3960 to 0.9435, respectively. Sixteen of 19 loci conformed to Hardy-Weinberg equilibrium, and no significant linkage disequilibrium was detected between any locus pairs. Our study supplies candidate microsatellite markers that can be useful for studying the population genetic structure of ovate pompano.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.December.12.13