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Nineteen polymorphic microsatellite markers developed for Trachinotus ovatus

To evaluate the population genetic diversity of the ovate pompano, we isolated and characterized 19 microsatellite markers using a (CA)13-enriched genomic library. Polymorphism was assessed in 30 individuals from a single population collected from the Daya Bay Aquaculture Center, Guangdong, China. T...

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Published in:	Genetics and molecular research 2014-12, Vol.13 (4), p.10518-10522
Main Authors:	Xie, Z Z, Huang, M W, Xu, W, Peng, C, He, J N, Meng, Z N, Zhang, Y, Li, S S, Lin, H R
Format:	Article
Language:	English
Subjects:	Alleles Animals China Fishes - genetics Genetic Variation Genetics, Population Linkage Disequilibrium Microsatellite Repeats - genetics Trachinotus ovatus
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creator	Xie, Z Z Huang, M W Xu, W Peng, C He, J N Meng, Z N Zhang, Y Li, S S Lin, H R
description	To evaluate the population genetic diversity of the ovate pompano, we isolated and characterized 19 microsatellite markers using a (CA)13-enriched genomic library. Polymorphism was assessed in 30 individuals from a single population collected from the Daya Bay Aquaculture Center, Guangdong, China. The number of alleles per locus ranged from 2 to 18 with an average of 7.8. The observed and expected heterozygosities varied from 0.2667 to 1.000 and from 0.3960 to 0.9435, respectively. Sixteen of 19 loci conformed to Hardy-Weinberg equilibrium, and no significant linkage disequilibrium was detected between any locus pairs. Our study supplies candidate microsatellite markers that can be useful for studying the population genetic structure of ovate pompano.
doi_str_mv	10.4238/2014.December.12.13
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subjects	Alleles Animals China Fishes - genetics Genetic Variation Genetics, Population Linkage Disequilibrium Microsatellite Repeats - genetics Trachinotus ovatus
title	Nineteen polymorphic microsatellite markers developed for Trachinotus ovatus
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