T cell receptor-mediated stimulation of mouse thymocytes induces up-regulation of the GM2/GD2 synthase gene

cDNA clones of the mouse GM2/GD2 synthase (EC 2.4.1.92) gene were isolated, and their analyses revealed that the protein has a type II transmembrane structure with 533 amino acids, which was very similar to the human homolog except for the mRNA size. The mRNA level in thymocytes dramatically increas...

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Bibliographic Details
Published in:FEBS letters 1995-01, Vol.358 (1), p.79-83
Main Authors: Takamiya, Kogo, Yamamoto, Akihito, Yamashiro, Shuji, Furukawa, Keiko, Haraguchi, Masashi, Okada, Masahiko, Ikeda, Takayoshi, Shiku, Hiroshi, Furukawa, Koichi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
CD3 Complex - physiology
Cloning, Molecular
Dinoprostone - pharmacology
DNA, Complementary - genetics
GalNAcβ1→4 (NeuAcα2→3) Galβ1→4 Glc-Cer
GalNAcβ1→4 (NeuAcα2→8NeuAcα2→3) Galβ1→4 Glc-Cer
Ganglioside
Ganglioside nomenclature is based on that of Svennerholm
GD2
Glycosyltransferase
GM2
Humans
immunolluorescence
kbp(s)
kilobasepair(s)
Lymphocyte Activation
mAb
Male
Mice
Mice, Inbred BALB C
Molecular Sequence Data
monoclonal antibody
N-Acetylgalactosamine
N-Acetylgalactosaminyltransferases - genetics
N-Acetylgalactosaminyltransferases - metabolism
PGE2
Polypeptide N-acetylgalactosaminyltransferase
prostaglandin E2
Receptors, Antigen, T-Cell - physiology
RNA, Messenger - biosynthesis
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
T cell receptor
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Thymocyte
Tumor Cells, Cultured
Up-Regulation - drug effects
Up-Regulation - physiology
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Description
Summary:cDNA clones of the mouse GM2/GD2 synthase (EC 2.4.1.92) gene were isolated, and their analyses revealed that the protein has a type II transmembrane structure with 533 amino acids, which was very similar to the human homolog except for the mRNA size. The mRNA level in thymocytes dramatically increased after treatment with anti-CD3 monoclonal antibody, whereas it was not elevated when treated with prostaglandin E2. In situ hybridization showed an elevation of mRNA levels in medullar thymocytes, suggesting that T cell receptor-mediated signaling induces up-regulation of the GM2/GD2 synthase gene in mature thymocytes.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)01395-H