Evidence for the presence of clofazimine and its distribution in the healthy mouse brain

This is the first report of clofazimine (CFZ) penetration and distribution in normal mouse brain. Mice were administered 25 mg/kg CFZ or 100 mg/kg CFZ orally, daily for 2 weeks. Animals were sacrificed and blood and brain tissues were harvested. Liquid chromatography tandem mass spectrometry showed...

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Bibliographic Details
Published in:Journal of molecular histology 2015-10, Vol.46 (4-5), p.439-442
Main Authors: Baijnath, Sooraj, Naiker, Suhashni, Shobo, Adeola, Moodley, Chivonne, Adamson, John, Ngcobo, Bongani, Bester, Linda A., Singh, Sanil, Kruger, Hendrik G., Naicker, Tricia, Govender, Thavendran
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - administration & dosage
Anti-Inflammatory Agents - pharmacokinetics
Biomedical and Life Sciences
Biomedicine
Brain - drug effects
Brain - metabolism
Cell Biology
Clofazimine - administration & dosage
Clofazimine - pharmacokinetics
Developmental Biology
Life Sciences
Mice
Short Communication
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
Tissue Distribution
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Summary:This is the first report of clofazimine (CFZ) penetration and distribution in normal mouse brain. Mice were administered 25 mg/kg CFZ or 100 mg/kg CFZ orally, daily for 2 weeks. Animals were sacrificed and blood and brain tissues were harvested. Liquid chromatography tandem mass spectrometry showed high concentrations of CFZ in homogenized brain, with 100 mg/kg dose having significantly higher concentration than 25 mg/kg. Matrix-assisted laser desorption/ionization spectrometric imaging of brain sections showed widespread tissue distribution of CFZ. Our results show dose dependent localization in brain.
ISSN:1567-2379
1567-2387
DOI:10.1007/s10735-015-9634-3