Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products

A multiplex PCR for the simultaneous detection of Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes sea, sec, sed, seg, seh, sei, sej, sel was developed. The method was used to determine the presence of enterotoxigenic types for 93 S. aureus stra...

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Bibliographic Details
Published in:Molecular and cellular probes 2005-10, Vol.19 (5), p.299-305
Main Authors: Cremonesi, Paola, Luzzana, Massimo, Brasca, Milena, Morandi, Stefano, Lodi, Roberta, Vimercati, Chiara, Agnellini, Dario, Caramenti, Giancarlo, Moroni, Paolo, Castiglioni, Bianca
Format: Article
Language:English
Subjects:
Animals
Dairy products
Dairy Products - microbiology
Enterotoxigenic Staphylococcus aureus
Enterotoxins
Food Contamination
Humans
Milk
Milk - microbiology
Multiplex PCR
Polymerase Chain Reaction - methods
Reproducibility of Results
RNA, Ribosomal, 23S - metabolism
Sensitivity and Specificity
Staphylococcus aureus
Staphylococcus aureus - classification
Staphylococcus aureus - genetics
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Summary:A multiplex PCR for the simultaneous detection of Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes sea, sec, sed, seg, seh, sei, sej, sel was developed. The method was used to determine the presence of enterotoxigenic types for 93 S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1 pg of DNA, showing the excellent specificity and high sensitivity of the assay.
ISSN:0890-8508
1096-1194
DOI:10.1016/j.mcp.2005.03.002