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Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products
A multiplex PCR for the simultaneous detection of Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes sea, sec, sed, seg, seh, sei, sej, sel was developed. The method was used to determine the presence of enterotoxigenic types for 93 S. aureus stra...
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| Published in: | Molecular and cellular probes 2005-10, Vol.19 (5), p.299-305 |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c382t-e4a2b29c48d38da287e60584574a0063fdb1941467dbb2aa9336c04cf0530de23 |
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| cites | cdi_FETCH-LOGICAL-c382t-e4a2b29c48d38da287e60584574a0063fdb1941467dbb2aa9336c04cf0530de23 |
| container_end_page | 305 |
| container_issue | 5 |
| container_start_page | 299 |
| container_title | Molecular and cellular probes |
| container_volume | 19 |
| creator | Cremonesi, Paola Luzzana, Massimo Brasca, Milena Morandi, Stefano Lodi, Roberta Vimercati, Chiara Agnellini, Dario Caramenti, Giancarlo Moroni, Paolo Castiglioni, Bianca |
| description | A multiplex PCR for the simultaneous detection of
Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes
sea,
sec,
sed,
seg,
seh,
sei,
sej,
sel was developed. The method was used to determine the presence of enterotoxigenic types for 93
S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some
se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1
pg of DNA, showing the excellent specificity and high sensitivity of the assay. |
| doi_str_mv | 10.1016/j.mcp.2005.03.002 |
| format | article |
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Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes
sea,
sec,
sed,
seg,
seh,
sei,
sej,
sel was developed. The method was used to determine the presence of enterotoxigenic types for 93
S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some
se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1
pg of DNA, showing the excellent specificity and high sensitivity of the assay.</description><identifier>ISSN: 0890-8508</identifier><identifier>EISSN: 1096-1194</identifier><identifier>DOI: 10.1016/j.mcp.2005.03.002</identifier><identifier>PMID: 16006095</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Dairy products ; Dairy Products - microbiology ; Enterotoxigenic Staphylococcus aureus ; Enterotoxins ; Food Contamination ; Humans ; Milk ; Milk - microbiology ; Multiplex PCR ; Polymerase Chain Reaction - methods ; Reproducibility of Results ; RNA, Ribosomal, 23S - metabolism ; Sensitivity and Specificity ; Staphylococcus aureus ; Staphylococcus aureus - classification ; Staphylococcus aureus - genetics</subject><ispartof>Molecular and cellular probes, 2005-10, Vol.19 (5), p.299-305</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-e4a2b29c48d38da287e60584574a0063fdb1941467dbb2aa9336c04cf0530de23</citedby><cites>FETCH-LOGICAL-c382t-e4a2b29c48d38da287e60584574a0063fdb1941467dbb2aa9336c04cf0530de23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16006095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cremonesi, Paola</creatorcontrib><creatorcontrib>Luzzana, Massimo</creatorcontrib><creatorcontrib>Brasca, Milena</creatorcontrib><creatorcontrib>Morandi, Stefano</creatorcontrib><creatorcontrib>Lodi, Roberta</creatorcontrib><creatorcontrib>Vimercati, Chiara</creatorcontrib><creatorcontrib>Agnellini, Dario</creatorcontrib><creatorcontrib>Caramenti, Giancarlo</creatorcontrib><creatorcontrib>Moroni, Paolo</creatorcontrib><creatorcontrib>Castiglioni, Bianca</creatorcontrib><title>Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products</title><title>Molecular and cellular probes</title><addtitle>Mol Cell Probes</addtitle><description>A multiplex PCR for the simultaneous detection of
Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes
sea,
sec,
sed,
seg,
seh,
sei,
sej,
sel was developed. The method was used to determine the presence of enterotoxigenic types for 93
S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some
se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1
pg of DNA, showing the excellent specificity and high sensitivity of the assay.</description><subject>Animals</subject><subject>Dairy products</subject><subject>Dairy Products - microbiology</subject><subject>Enterotoxigenic Staphylococcus aureus</subject><subject>Enterotoxins</subject><subject>Food Contamination</subject><subject>Humans</subject><subject>Milk</subject><subject>Milk - microbiology</subject><subject>Multiplex PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reproducibility of Results</subject><subject>RNA, Ribosomal, 23S - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - classification</subject><subject>Staphylococcus aureus - genetics</subject><issn>0890-8508</issn><issn>1096-1194</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAUhS0EokPhAdggr9glXMdOxhErNPxKlYpaWFuOfUM9OHGwnarzGjwxHs1I7Lo6m--eq3MOIa8Z1AxY925fT2apG4C2Bl4DNE_IhkHfVYz14inZgOyhki3IC_IipT0A9ALkc3LBOoAO-nZD_n7Ee_RhmXDONIxU02n12S0eH-j33Q3VKekDHUOk-Q6pswVzozM6uzAf-dusl7uDDyYYsyaq14hFCoUx5PDgfuHsDE05ajcn6lLwOqOlYwwTnZz_TfVsqdUuHugSg11NTi_Js1H7hK_Oekl-fv70Y_e1urr-8m334aoyXDa5QqGboemNkJZLqxu5xQ5aKdqt0CUdH-1QWmCi29phaLTuOe8MCDNCy8Fiwy_J25NvefxnxZTV5JJB7_WMYU2KbaFtOyYKyE6giSGliKNaopt0PCgG6jiE2qsyhDoOoYCrMkS5eXM2X4cJ7f-Lc_MFeH8CsES8dxhVMg5ng9ZFNFnZ4B6x_wd3bJwc</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Cremonesi, Paola</creator><creator>Luzzana, Massimo</creator><creator>Brasca, Milena</creator><creator>Morandi, Stefano</creator><creator>Lodi, Roberta</creator><creator>Vimercati, Chiara</creator><creator>Agnellini, Dario</creator><creator>Caramenti, Giancarlo</creator><creator>Moroni, Paolo</creator><creator>Castiglioni, Bianca</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20051001</creationdate><title>Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products</title><author>Cremonesi, Paola ; Luzzana, Massimo ; Brasca, Milena ; Morandi, Stefano ; Lodi, Roberta ; Vimercati, Chiara ; Agnellini, Dario ; Caramenti, Giancarlo ; Moroni, Paolo ; Castiglioni, Bianca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-e4a2b29c48d38da287e60584574a0063fdb1941467dbb2aa9336c04cf0530de23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Dairy products</topic><topic>Dairy Products - microbiology</topic><topic>Enterotoxigenic Staphylococcus aureus</topic><topic>Enterotoxins</topic><topic>Food Contamination</topic><topic>Humans</topic><topic>Milk</topic><topic>Milk - microbiology</topic><topic>Multiplex PCR</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reproducibility of Results</topic><topic>RNA, Ribosomal, 23S - metabolism</topic><topic>Sensitivity and Specificity</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - classification</topic><topic>Staphylococcus aureus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cremonesi, Paola</creatorcontrib><creatorcontrib>Luzzana, Massimo</creatorcontrib><creatorcontrib>Brasca, Milena</creatorcontrib><creatorcontrib>Morandi, Stefano</creatorcontrib><creatorcontrib>Lodi, Roberta</creatorcontrib><creatorcontrib>Vimercati, Chiara</creatorcontrib><creatorcontrib>Agnellini, Dario</creatorcontrib><creatorcontrib>Caramenti, Giancarlo</creatorcontrib><creatorcontrib>Moroni, Paolo</creatorcontrib><creatorcontrib>Castiglioni, Bianca</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Molecular and cellular probes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cremonesi, Paola</au><au>Luzzana, Massimo</au><au>Brasca, Milena</au><au>Morandi, Stefano</au><au>Lodi, Roberta</au><au>Vimercati, Chiara</au><au>Agnellini, Dario</au><au>Caramenti, Giancarlo</au><au>Moroni, Paolo</au><au>Castiglioni, Bianca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products</atitle><jtitle>Molecular and cellular probes</jtitle><addtitle>Mol Cell Probes</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>19</volume><issue>5</issue><spage>299</spage><epage>305</epage><pages>299-305</pages><issn>0890-8508</issn><eissn>1096-1194</eissn><abstract>A multiplex PCR for the simultaneous detection of
Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes
sea,
sec,
sed,
seg,
seh,
sei,
sej,
sel was developed. The method was used to determine the presence of enterotoxigenic types for 93
S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some
se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1
pg of DNA, showing the excellent specificity and high sensitivity of the assay.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16006095</pmid><doi>10.1016/j.mcp.2005.03.002</doi><tpages>7</tpages></addata></record> |
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| ispartof | Molecular and cellular probes, 2005-10, Vol.19 (5), p.299-305 |
| issn | 0890-8508 1096-1194 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17055614 |
| source | ScienceDirect Freedom Collection |
| subjects | Animals Dairy products Dairy Products - microbiology Enterotoxigenic Staphylococcus aureus Enterotoxins Food Contamination Humans Milk Milk - microbiology Multiplex PCR Polymerase Chain Reaction - methods Reproducibility of Results RNA, Ribosomal, 23S - metabolism Sensitivity and Specificity Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - genetics |
| title | Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products |
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