Prolonged exposure to lidocaine disturbs preimplantation mouse embryo development

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 1996-03, Vol.10 (2), p.113-118
• Main Authors: Del Valle, Luis J., Orihuela, Pedro A.
• Format: Article
• Language: English
• Subjects: anesthetics; Animals; Biological and medical sciences; Drug Administration Schedule; Drug toxicity and drugs side effects treatment; early development; Embryonic and Fetal Development - drug effects; Embryonic Development - drug effects; Female; lidocaine; Lidocaine - administration & dosage; Lidocaine - toxicity; Litter Size - drug effects; Medical sciences; Mice; Miscellaneous (drug allergy, mutagens, teratogens...); Organ Culture Techniques; Pharmacology. Drug treatments; Pregnancy; preimplantation embryo; reproductive risk
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/0890-6238(95)02052-7

Objetive: To determine the in vitro and in vivo effects of lidocaine on preimplantation mouse embryo development during prolonged exposure. Study design: In vitro experiments: Two-cell mouse embryos were exposed to lidocaine doses of 0,10,100, and 1000 μg/mL for 72 h. In vivo experiments, female mice were exposed to lidocaine doses of 0,0.3,1.7, and 3.3 mg/kg body weight on days 1,2,3, and 4 of pregnancy. Early development, cell number, mitotic index, and micronuclei frequency were examined. Results: Development to the blastocyst stage was inhibited by all the doses tested in vitro and in vivo. Most of the affected embryos showed arrest and degeneration. The cell number was decreased, but not the mitotic index. Furthermore, clastogenic damage was observed in vitro. Conclusions: Lidocaine adversely affects preimplantation mouse embryo development in vitro and in vivo.