Evaluation of technical HCH residues in differentiating rat intestinal epithelial cells

• Published in: Bulletin of environmental contamination and toxicology 1996-04, Vol.56 (4), p.617-621
• Main Authors: SHUKLA, S, UPRETI, R. K, RAIZADA, R. B, SRIVASTAVA, M. K, KIDWAI, A. M
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Biological and medical sciences; Cell Differentiation; cells; cellule; celulas; Chromatography, Gas; diferenciacion celular; differenciation cellulaire; Epithelial Cells; Epithelium - drug effects; Epithelium - metabolism; hch; Hexachlorocyclohexane - administration & dosage; Hexachlorocyclohexane - analysis; Hexachlorocyclohexane - metabolism; Hexachlorocyclohexane - toxicity; insecticidas; insecticide; insecticides; Insecticides - administration & dosage; Insecticides - analysis; Insecticides - metabolism; Insecticides - toxicity; intestin; Intestine, Small - cytology; Intestine, Small - drug effects; Intestine, Small - metabolism; intestines; intestinos; Male; Medical sciences; membrana mucosa; mucous membrane; muqueuse; Pesticide Residues - analysis; Pesticides, fertilizers and other agrochemicals toxicology; rat; rata; Rats; residu; residues; residuos; Stereoisomerism; Toxicology
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s001289900089

Technical HCH (hexachlorocyclohexane) is a mixture of different isomers and its major insecticidal activity is due to the gamma-isomer. HCH have been shown to accumulate in fat depots after ingestion. The accumulation of HCH causes the disturbances of lipid metabolism. Membranes are target sites for immediate and delayed action of HCH and are known to play a significant role in the mobilization of lipids from tissues to plasma. Gamma HCH alters the activity of some membrane bound proteins and the membrane permeability. HCH is absorbed from GI-tract, skin and lungs. A large amount of HCH isomers have been shown to be absorbed through GI-tract. However, not much literature is available on the effect of technical HCH and its metabolites on rat intestinal epithelial cell lining. Although HCH is stored in fatty tissues, the intestinal cell lining is the first target site of its contact following oral administration. Present investigation deals with the analysis of HCH-isomers in differentiating intestinal epithelial cells. The residual levels of HCH isomers in the intestinal epithelial cells would be important in understanding their residual effect on function and differentiation of epithelial cells.