Th-17 cells infiltrate the liver in human biliary atresia and are related to surgical outcome

• Published in: Journal of pediatric surgery 2015-08, Vol.50 (8), p.1297-1303
• Main Authors: Hill, Richard, Quaglia, Alberto, Hussain, Munther, Hadzic, Nedim, Mieli-Vergani, Giorgina, Vergani, Diego, Davenport, Mark
• Format: Article
• Language: English
• Subjects: Biliary atresia; Biliary Atresia - immunology; Biliary Atresia - surgery; Biliary Atresia - virology; Biomarkers - metabolism; Case-Control Studies; CD4-Positive T-Lymphocytes - metabolism; Humans; Infant; Infant, Newborn; Inflammatory response; Interleukin-17; Liver - immunology; Liver - surgery; Liver - virology; Pediatrics; Portoenterostomy, Hepatic; Surgery; T-cells; Th17 Cells - metabolism; Treatment Outcome; Tregs
• ISSN: 0022-3468; 1531-5037
• DOI: 10.1016/j.jpedsurg.2015.02.005

Abstract Background Biliary atresia (BA), a cholangiopathy of unknown etiology is associated with intrahepatic mononuclear cell infiltrate. An abnormal reaction to viral exposure has been hypothesized in some cases. We aimed to investigate the nature of the CD4 + hepatic infiltrate in defined clinical variants of BA by quantification of inflammatory cell components. Methods Liver biopsies of infants obtained at Kasai portoenterostomy (KPE) were stained immunohistochemically using monoclonal antibodies to Tbet, GATA-3, FOXP3 and interleukin (IL) 17, identifying Th-1, Th-2, Tregs and Th-17 cells respectively. T cells were counted with the aid of a graticule. Data are reported as median (range) of cells per high-power-field (× 400) and compared using nonparametric statistical tests with P ≤ 0.05 regarded as significant. Results Liver biopsies from BA (n = 37) and age-matched cholestatic controls (e.g. alpha-1-anti trypsin deficiency, Alagilles syndrome, n = 12) were investigated. BA infants were divided into three groups: cytomegalovirus IgM + ve (CMV; n = 9); BA splenic malformation (BASM; n = 9) and isolated BA (IBA; n = 19). All T-cell subsets were present in the portal tracts, with an overrepresentation of Th-1 (P < 0.001) and Th-17 (P < 0.03), but not Th-2 (P = 0.94) or Tregs (P = 0.15), compared to controls. Th-1 cells predominated in the CMV group; (18 [7–37] vs. 3 [0–14] [BASM] and vs. 5 [3–23] [IBA]; P < 0.01 both), while no subgroup differences were seen for Th-17 cells. The degree of Th-1 cell infiltrate inversely correlated with platelet count (rS = − 0.49; P < 0.01). Th-17 cells were fewer (6 [2–11] vs. 11 [8–20]; P = 0.02) in infants who cleared their jaundice (n = 15, < 20 μmol/L) although this did not translate to improved native liver survival (P = 0.17). Conclusions Th-17 cells infiltrate the liver in BA and are associated with a worse surgical outcome; a Th-1 profile predominates in CMV-associated BA.