Thermo-chemotherapy Induced miR-218 upregulation inhibits the invasion of gastric cancer via targeting Gli2 and E-cadherin

• Published in: Tumor biology 2015-08, Vol.36 (8), p.5807-5814
• Main Authors: Ruan, Qiang, Fang, Zhi-yuan, Cui, Shu-zhong, Zhang, Xiang-liang, Wu, Yin-bing, Tang, Hong-sheng, Tu, Yi-nuo, Ding, Yan
• Format: Article
• Language: English
• Subjects: Adult; Aged; Biomedical and Life Sciences; Biomedicine; Cadherins - genetics; Cancer Research; Cell adhesion & migration; Cell Proliferation - drug effects; Chemotherapy; Clinical outcomes; Epithelial-Mesenchymal Transition - drug effects; Female; Gastric cancer; Gene Expression Regulation, Neoplastic - drug effects; Humans; Hyperthermia, Induced; Kruppel-Like Transcription Factors - genetics; Lymphatic Metastasis; Male; MicroRNAs - biosynthesis; MicroRNAs - genetics; Middle Aged; Neoplasm Invasiveness - genetics; Nuclear Proteins - genetics; Protein expression; Research Article; Stomach Neoplasms - drug therapy; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; Zinc Finger Protein Gli2
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1007/s13277-015-3250-4

Thermo-chemotherapy has been proven to reduce the invasion capability of cancer cells. However, the molecular mechanism underlying this anti-invasion effect is still unclear. In this study, the role of thermo-chemotherapy in the inhibition of tumor invasion was studied. The results demonstrated that expression of miR-218 was downregulated in gastric cancer tissues, which had a positive correlation with tumor invasion and metastasis. In vitro thermo-chemotherapy increased miR-218 expression in SGC7901 cells and inhibited both proliferation and invasion of cancer cells. Gli2 was identified as a downstream target of miR-218, and its expression was negatively regulated by miR-218. The thermo-chemotherapy induced miR-218 upregulation was also accompanied by increasing of E-cadherin expression. In conclusion, the present study indicates that thermo-chemotherapy can effectively decrease the invasion capability of cancer cells and increase cell-cell adhesion. miR-218 and its downstream target Gli2, as well as E-cadherin, participate in the anti-invasion process.