enzyme-immobilized microplate for determination of linamarin for large number of samples

An enzyme-immobilized microplate for determination of linamarin was prepared by covalently linking cassava leaf linamarase to the microplate. For linamarin determination, cassava roots were homogenised in 0.1M o-phosphoric acid and the filtrate adjusted to pH 6 with NaOH prior to adding into the wel...

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Bibliographic Details
Published in:Biotechnology techniques 1994-05, Vol.8 (5), p.337-338
Main Authors: Yeoh, H.H, Tan, C.K.C
Format: Article
Language:English
Subjects:
analytical methods
anions
Biological and medical sciences
Biotechnology
cassava
cyanide
enzyme activity
enzymes
Fundamental and applied biological sciences. Psychology
hydrolysis
Immobilization of enzymes and other molecules
Immobilization techniques
linamarase
linamarin
Manihot esculenta
measurement
Methods. Procedures. Technologies
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Summary:An enzyme-immobilized microplate for determination of linamarin was prepared by covalently linking cassava leaf linamarase to the microplate. For linamarin determination, cassava roots were homogenised in 0.1M o-phosphoric acid and the filtrate adjusted to pH 6 with NaOH prior to adding into the wells. The cyanide released was then determined spectrophotometrically. One nmol linamarin can be detected. The microplate method is suitable for analysis of large number of samples and is useful for screening purposes.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF02428977