Selection of bacterial antagonists using enrichment cultures for the control of summer patch disease in Kentucky bluegrass

A continuous enrichment culture procedure was used to isolate bacteria from various soil sources capable of suppressing summer patch disease of turfgrass. Cultures consisted of a minimal salts medium supplemented with the mycelium of Magnaporthe poae, the causal agent of summer patch, as the sole ca...

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Bibliographic Details
Published in:Current microbiology 1996-02, Vol.32 (2), p.106-110
Main Authors: Kobayashi, D.Y. (State University of New Jersey, Cook College, New Brunswick, NJ.), El-Barrad, N.E.H
Format: Article
Language:English
Subjects:
BACTERIA
Biological and medical sciences
Biological control
Control
CONTROL DE ENFERMEDADES
CONTROLE DE MALADIES
ENFERMEDADES FUNGOSAS
FLORA DEL SUELO
FLORE DU SOL
FONGICIDE
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
FUNGICIDAS
MAGNAPORTHE
MALADIE FONGIQUE
PESTICIDE MICROBIEN
Phytopathology. Animal pests. Plant and forest protection
PLAGUICIDAS MICROBIANOS
POA PRATENSIS
RHIZOSPHERE
RIZOSFERA
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Summary:A continuous enrichment culture procedure was used to isolate bacteria from various soil sources capable of suppressing summer patch disease of turfgrass. Cultures consisted of a minimal salts medium supplemented with the mycelium of Magnaporthe poae, the causal agent of summer patch, as the sole carbon source. Percentages of chitinolytic bacteria increased in all enrichments over time. Approximately 30% of randomly selected isolates from enrichment cultures significantly reduced summer patch symptom development compared with untreated controls. Six isolates consistently suppressed summer patch in Kentucky bluegrass by more than 50% compared with controls in repeated experiments. All six isolates colonized the turfgrass rhizosphere in high populations. The six isolates ranged in the spectrum of extracellular enzymes that they expressed. The best disease-suppressing isolate, N4-7, expressed chitinase, glucanase, lipase, and protease activity, and inhibited the growth of M. poae in vitro
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900019