Clinical evaluation of repetitive sequence-based polymerase chain reaction using the DiversiLab System for strain typing of vancomycin-resistant enterococci

The reliability of the DiversiLab System, an automated method of microbial strain typing using repetitive sequence-based polymerase chain reaction (rep-PCR), was evaluated by comparing results with those obtained by pulsed-field gel electrophoresis (PFGE). Ninety-five clinical isolates of vancomycin...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2006-03, Vol.54 (3), p.183-187
Main Authors: Pounder, June I., Shutt, Cheryl K., Schaecher, Barbara J., Woods, Gail L.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
DiversiLab System
Enterococcus
Fundamental and applied biological sciences. Psychology
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Repetitive sequence-based PCR
Vancomycin-resistant enterococci
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Summary:The reliability of the DiversiLab System, an automated method of microbial strain typing using repetitive sequence-based polymerase chain reaction (rep-PCR), was evaluated by comparing results with those obtained by pulsed-field gel electrophoresis (PFGE). Ninety-five clinical isolates of vancomycin-resistant enterococci (VRE; 13 groups, 2–17 isolates per group) sent to Associated Regional and University Pathologists (ARUP) Laboratories for typing were tested by both methods. Rep-PCR and PFGE results were concordant for 83 isolates: all 32 isolates in 6 of the groups and 51 of the 63 isolates in the other 7 groups. Clustering of the remaining 12 isolates differed. With the DiversiLab System, analysis is objective, and results are available in 4 h, compared with a more subjective analysis and a 2- to 3-day turnaround time for PFGE. The DiversiLab System may be a viable alternative to PFGE for typing VRE in clinical reference laboratories.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2005.08.004