Interaction between vitamin B6 and source of selenium on the response of the selenium-dependent glutathione peroxidase system to oxidative stress induced by oestrus in pubertal pig

• Published in: Journal of trace elements in medicine and biology 2015-10, Vol.32, p.21-29
• Main Authors: Dalto, Danyel Bueno, Roy, Mélanie, Audet, Isabelle, Palin, Marie-France, Guay, Frédéric, Lapointe, Jérôme, Matte, J. Jacques
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Diet; Estrus - drug effects; Female; Gene Expression Regulation - drug effects; Gilt; Glutathione peroxidase; Glutathione Peroxidase - blood; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Inorganic selenium; Kidney - drug effects; Kidney - enzymology; Liver - drug effects; Liver - enzymology; Luteinizing Hormone - metabolism; Metabolome - drug effects; Oestrus; Organic selenium; Ovulation - drug effects; Oxidative Stress - drug effects; Puberty - drug effects; Pyridoxal Phosphate - metabolism; Pyridoxine; RNA, Messenger - genetics; RNA, Messenger - metabolism; Selenium - blood; Selenium - pharmacology; Time Factors; Uterus - drug effects; Uterus - metabolism; Vitamin B 6 - pharmacology
• ISSN: 0946-672X; 1878-3252
• DOI: 10.1016/j.jtemb.2015.05.002

•Dietary Se affected long-term Se status but not glutathione peroxidase activity.•Other antioxidation systems were not affected by dietary Se and vitamin B6.•Peri-oestrus blood glutathione peroxidase activity was affected by dietary Se.•Dietary Se and vitamin B6 maximized mRNA glutathione peroxidase after oestrus.•Peri-oestrus period is a useful oxidation model for glutathione peroxidase studies. This study aimed to assess the interaction between vitamin B6 and selenium (Se) for the flow of Se towards the Se-dependent glutathione peroxidase (GPX) system in response to oxidative stress naturally induced by oestrus in a pubertal pig model. At first oestrus, forty-five gilts were randomly assigned to the experimental diets (n=9/group): basal diet (CONT); CONT+0.3mg/kg of Na-selenite (MSeB60); MSeB60+10mg/kg of HCl-B6 (MSeB610); CONT+0.3mg/kg of Se-enriched yeast (OSeB60); and OSeB60+10mg/kg of HCl-B6 (OSeB610). Blood samples were collected at each oestrus (long-term profiles), and daily from day −4 to +3 (slaughter) of the fourth oestrus (peri-oestrus profiles) after which liver, kidneys, and ovaries were collected. For long-term profiles, CONT had lower blood Se than Se-supplemented gilts (p