Manipulating DNA molecules in nanofluidic channels

Nanofluidic channels of similar to 40x60 nm (width x depth) were fabricated with focused ion beam (FIB) milling instrument on a silicon nitride (Si sub(3)N sub(4)) film. Stained lambda -phage DNA molecules were driven into these open channels by capillary force and observed with fluorescence microsc...

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Bibliographic Details
Published in:Microfluidics and nanofluidics 2006-01, Vol.2 (1), p.85-88
Main Authors: Wang, K-G, Yue, S, Wang, L, Jin, A, Gu, C, Wang, P-Y, Feng, Y, Wang, Y, Niu, H
Format: Article
Language:English
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Summary:Nanofluidic channels of similar to 40x60 nm (width x depth) were fabricated with focused ion beam (FIB) milling instrument on a silicon nitride (Si sub(3)N sub(4)) film. Stained lambda -phage DNA molecules were driven into these open channels by capillary force and observed with fluorescence microscopy. The movements of DNA molecule in these channels were discussed. These sub-100 nm scale channels may be useful in studying single biomacromolecules.
ISSN:1613-4982
DOI:10.1007/s10404-005-0057-4