Upregulation of Collagen Expression via PPARβ/δ Activation in Aged Skin by Magnesium Lithospermate B from Salvia miltiorrhiza

This study investigated the agonistic activity of magnesium lithospermate B (1), isolated from Salvia miltiorrhiza, on peroxisome proliferator-activated receptor (PPARβ/δ) and the expressions of collagen genes (COL1A1 and COL3A1) and transforming growth factor-β1 (TGF-β1) in models of skin aging. Th...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2015-08, Vol.78 (8), p.2110-2115
Main Authors: Jung, Yu Ri, Lee, Eun Kyeong, Kim, Dae Hyun, Park, Chan Hum, Park, Min Hi, Jeong, Hyoung Oh, Yokozawa, Takako, Tanaka, Takashi, Im, Dong Soon, Kim, Nam Deuk, Yu, Byung Pal, Mo, Sang Hyun, Chung, Hae Young
Format: Article
Language:English
Subjects:
Aged
Animals
Collagen - metabolism
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - metabolism
Fibroblasts - drug effects
Humans
Magnesium - metabolism
Male
Molecular Structure
NF-kappa B - metabolism
PPAR delta - metabolism
PPAR-beta - metabolism
Rats
Rats, Sprague-Dawley
Salvia miltiorrhiza - chemistry
Skin - drug effects
Transcriptional Activation
Up-Regulation
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Summary:This study investigated the agonistic activity of magnesium lithospermate B (1), isolated from Salvia miltiorrhiza, on peroxisome proliferator-activated receptor (PPARβ/δ) and the expressions of collagen genes (COL1A1 and COL3A1) and transforming growth factor-β1 (TGF-β1) in models of skin aging. The action of compound 1 as a PPARβ/δ agonist was determined by reporter gene assay, immunostaining, and Western blotting. To determine the antiaging effects of compound 1 on skin, aged Sprague–Dawley rat skin and ultraviolet B (UVB)-irradiated human skin fibroblasts were used. The results show that 1 presented a marked enhancement of both nuclear protein levels and activity of PPARβ/δ in fibroblasts. In addition, 1 prevented downregulation of PPARβ/δ activity in aged rat skin and UVB-induced fibroblasts. Furthermore, 1 increased the expressions of COL1A1, COL3A1, and TGF-β1 in vivo and in a cell culture system. Therefore, the present study shows that compound 1 prevents collagen degradation in aged rat skin and UVB-exposed fibroblasts through PPARβ/δ activation. The therapeutic and cosmetic applications of compound 1 need further investigation.
ISSN:0163-3864
1520-6025
DOI:10.1021/acs.jnatprod.5b00348