Effects of corticosteroids and hyaluronic acid on torn rotator cuff tendons in vitro and in rats

ABSTRACT Corticosteroids (CS) or hyaluronic acid (HA) is used in subacromial injection for the conservative treatment of rotator cuff tears (RCT); this study addresses the question of how CS and HA affect the tendon tissue and fibroblasts in vitro and in rats. Cell proliferation assays were performe...

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Bibliographic Details
Published in:Journal of orthopaedic research 2015-10, Vol.33 (10), p.1523-1530
Main Authors: Nakamura, Hidehiro, Gotoh, Masafumi, Kanazawa, Tomonoshin, Ohta, Keisuke, Nakamura, Keiichirou, Honda, Hirokazu, Ohzono, Hiroki, Shimokobe, Hisao, Mitsui, Yasuhiro, Shirachi, Isao, Okawa, Takahiro, Higuchi, Fujio, Shirahama, Masahiro, Shiba, Naoto, Matsueda, Satoko
Format: Article
Language:English
Subjects:
Adrenal Cortex Hormones - adverse effects
Animals
Cell Proliferation - drug effects
Cell Survival - drug effects
corticosteroids
Fibroblasts - drug effects
Fibroblasts - ultrastructure
Humans
Hyaluronic Acid - adverse effects
hyaluronic acids
Immunohistochemistry
Male
Random Allocation
Rats, Sprague-Dawley
Rotator Cuff - drug effects
Rotator Cuff Injuries
tendon
tendon fibroblast
totator cuff tears
Weight-Bearing
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Summary:ABSTRACT Corticosteroids (CS) or hyaluronic acid (HA) is used in subacromial injection for the conservative treatment of rotator cuff tears (RCT); this study addresses the question of how CS and HA affect the tendon tissue and fibroblasts in vitro and in rats. Cell proliferation assays were performed in human tendon fibroblasts from RCT. Rats underwent surgery to create RCT, and the surgical sites were injected with CS or HA. The rotator cuff tendons were subjected to biomechanical testing, microscopic and immunohistochemical analysis of proliferating cell nuclear antigen (PCNA), and ultrastructural analysis. Cell proliferation was significantly decreased with CS in vitro (p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.22921