Use of adjuvanted vaccines to lengthen the protection against lactococcosis in rainbow trout ( Oncorhynchus mykiss)

• Published in: Aquaculture 2006-02, Vol.251 (2), p.153-158
• Main Authors: Ravelo, Carmen, Magariños, Beatriz, Herrero, M. Carmen, Costa, Llorenç, Toranzo, Alicia E., Romalde, Jesús L.
• Format: Article
• Language: English
• Subjects: Adjuvanted vaccines; Animal aquaculture; animal pathogenic bacteria; Animal productions; Aquaculture; Bacteria; bacterial infections; Biological and medical sciences; Comparative analysis; disease prevention; Fish; fish culture; fish diseases; Freshwater; Fundamental and applied biological sciences. Psychology; General aspects; Lactococcus garvieae; mortality; Oncorhynchus mykiss; Rainbow trout; trout; Vaccination; vaccine adjuvants; Vaccines
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2005.05.027

In this work, we evaluated the effect of the inclusion of different adjuvants in the vaccine formulation against Lactococcus garvieae in comparison with an aqueous bacterin. The aqueous and non mineral oil adjuvanted vaccines (Montanide-ISA-763-A and Aquamun) yielded a good protection 4 weeks after the immunization (RPS of 82.6, 84.8 and 86.9, respectively). The protective rates obtained for the adjuvants Montanide-IMS-2212 (water based nanoparticles with immunostimulant) and Carbomer (high molecular weight polymer organic of polyacrylic acid) were 45.7% and 56.5%, respectively. We also evaluated the duration of protection confered by Aquamun adjuvanted-vaccine in comparison with the aqueous bacterin. Three months after vaccination, the RPS values obtained with the aqueous vaccine was 40% whereas with the Aquamun adjuvanted-vaccine the protection increased to values of 92%. In addition, a long lasting protection was observed with this adjuvanted vaccine with RPS values of 90% and 83.3% in the challenges performed at 6 and 8 months post-vaccination, respectively.