Diphenyl diselenide and 2,3-dimercaptopropanol increase the PTZ-induced chemical seizure and mortality in mice

• Published in: Brain research bulletin 2006-02, Vol.68 (6), p.414-418
• Main Authors: Brito, Verônica B., Folmer, Vanderlei, Puntel, Gustavo O., Fachinetto, Roselei, Soares, Júlio C.M., Zeni, Gilson, Nogueira, Cristina W., Rocha, João B.T.
• Format: Article
• Language: English
• Subjects: (PhSe) 2; Animals; BAL; Benzene Derivatives - toxicity; Brain - drug effects; Brain - metabolism; Brain - physiopathology; Chelating Agents - toxicity; Convulsants - toxicity; Dimercaprol - toxicity; Disease Models, Animal; Dose-Response Relationship, Drug; Drug Synergism; GABA; GABA Antagonists - toxicity; gamma-Aminobutyric Acid - metabolism; Glutamate; Male; Mice; Neural Inhibition - drug effects; Neural Inhibition - physiology; Organoselenium Compounds - toxicity; Pentylenetetrazole - toxicity; PTZ; Reaction Time - drug effects; Reaction Time - physiology; Receptors, GABA - drug effects; Receptors, GABA - metabolism; Seizure; Seizures - chemically induced; Seizures - metabolism; Seizures - physiopathology; Survival Rate; Time Factors
• ISSN: 0361-9230; 1873-2747
• DOI: 10.1016/j.brainresbull.2005.09.007

The aim of the present study was to evaluate the interaction between a classic GABAergic antagonist — pentylenetetrazol (PTZ) with an organoselenium compound — diphenyl diselenide (PhSe) 2 and with the metal chelating agent — 2,3 dimercaptopropanol (BAL). Mice were pre-treated with 150 μmol/kg (PhSe) 2 or BAL (250, 500 or 1000 μmol/kg) before treatment with PTZ. Pre-treatment with (PhSe) 2 reduced the latency for PTZ-induced seizure at doses of 40 and 60 mg/kg and cause a decrease in the latency for PTZ-induced death at the dose of 60 mg/kg. However, treatment with PTZ at dose of 80 mg/kg was not affected by (PhSe) 2 pre-treatment. Pre-treatment with BAL reduced the latency for PTZ-induced seizure at doses of 40 and 50 mg/kg. In addition, the latency for PTZ-induced death at the dose of 40 mg/kg was decreased significantly by pre-treatment with all doses of BAL. At the dose of 50 mg/kg, a significant decrease in the latency for death occurred only in mice pre-treated with 500 and 1000 μmol/kg of BAL. Our results indicate that the PTZ-induced chemical seizures and mortality was enhanced by (PhSe) 2 and BAL. These results indicated that (PhSe) 2 and BAL interact with PTZ possibly by modulating the GABAergic system.