Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering

In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC). CHS was prepared from squid pens by alkaline N-deacetylation. HA and β-TCP were extracted from mussel shells u...

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Bibliographic Details
Published in:International journal of biological macromolecules 2015-09, Vol.80, p.445-454
Main Authors: Shavandi, Amin, Bekhit, Alaa El-Din A., Ali, M. Azam, Sun, Zhifa
Format: Article
Language:English
Subjects:
Adsorption
Animal Shells - chemistry
Animals
Bivalvia - chemistry
Bone Substitutes - chemistry
Brachyura - chemistry
Chitosan
Chitosan - chemistry
Chitosan - isolation & purification
Compressive Strength
Decapodiformes - chemistry
Hydrogen-Ion Concentration
Hydroxyapatite
Molecular Mimicry
Porosity
Squid pen
Tissue Engineering
Tissue Scaffolds - chemistry
Water - chemistry
X-Ray Diffraction
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Summary:In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC). CHS was prepared from squid pens by alkaline N-deacetylation. HA and β-TCP were extracted from mussel shells using a microwave irradiation method. Two different composites were prepared by incorporating 50% (w/w) HA/(β-TCP) in CHS or CHC followed by lyophilization and cross-linking of composites by tripolyphosphate (TPP). The effect of different freezing temperatures of −20, −80 and −196°C on the physicochemical characteristics of composites was investigated. A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS. Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated. The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties. The results of this study indicated that composites produced at −20°C had higher mechanical properties and lower degradation rate compared with −80°C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2015.07.012