Renoprotective mechanisms of morin in cisplatin-induced kidney injury

• Published in: International immunopharmacology 2015-09, Vol.28 (1), p.500-506
• Main Authors: Wei, Zhengkai, He, Xuexiu, Kou, Jinhua, Wang, Jingjing, Chen, Libin, Yao, Minjun, Zhou, Ershun, Fu, Yunhe, Guo, Changming, Yang, Zhengtao
• Format: Article
• Language: English
• Subjects: Acute Kidney Injury - chemically induced; Acute Kidney Injury - drug therapy; Acute Kidney Injury - metabolism; Acute Kidney Injury - pathology; Animals; Antineoplastic Agents - adverse effects; Apoptosis; Apoptosis - drug effects; Blood Urea Nitrogen; Cisplatin; Cisplatin - adverse effects; Creatinine - blood; Flavonoids - pharmacology; Flavonoids - therapeutic use; Glutathione Peroxidase - metabolism; Inflammation; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Kidney injury; Male; Mice, Inbred BALB C; Morin; Oxidative stress; Oxidative Stress - drug effects; Protective Agents - pharmacology; Protective Agents - therapeutic use; Superoxide Dismutase - metabolism
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2015.07.009

In this study, we investigated the renoprotective effects of morin on cisplatin-induced kidney injury in mice. Serum creatinine and blood urea nitrogen (BUN) levels, glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activities were determined according to the corresponding kits. The mRNA levels of TNF-α and IL-1β in kidney tissues were measured by quantitative real-time PCR (qRT-PCR). The activities of cytochrome P450 2E1 (CYP2E1), nuclear factor kappa B (NF-κB) p65, P38 mitogen-activated protein kinase (MAPK), Bax, p53 and cleaved caspase 3 were evaluated by western blotting. The results showed that the model of cisplatin-induced kidney injury was successfully replicated, and morin significantly attenuated histopathological changes and decreased the levels of TNF-α and IL-1β in the kidneys. In addition, morin attenuated the activation of CYP2E1, phospho-NF-κB p65, phospho-P38 MAPK, Bax, phospho-p53 and cleaved caspase 3 in CP-induced kidney injury. In conclusion, these results indicated that the renoprotective mechanisms of morin may be attributed to the suppression of oxidative stress, inflammation and apoptosis in CP-induced kidney injury. •Morin decreased the cisplatin-induced serum creatinine and BUN levels.•Morin inhibited the cisplatin-induced TNF-α and IL-1β production.•Morin inhibited the activation of CYP2E1, p65, P38, Bax, p53 and cleaved caspase 3.•Morin attenuated cisplatin-induced oxidative stress, inflammation and apoptosis.