A novel type of matrix for surface-assisted laser desorption–ionization mass spectrometric detection of biomolecules using metal-organic frameworks

A 3D metal-organic framework (MOF) nanomaterial as matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and tandem mass spectrometry (MS/MS) was developed for the analysis of complex biomolecules. Unlike other nanoparticle matrices, this MOF nanomaterial does not need...

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Bibliographic Details
Published in:Analytica chimica acta 2015-08, Vol.888, p.103-109
Main Authors: Fu, Chien-Ping, Lirio, Stephen, Liu, Wan-Ling, Lin, Chia-Her, Huang, Hsi-Ya
Format: Article
Language:English
Subjects:
Biomolecules
Carbohydrates
Disaccharidases - analysis
Metal-organic frameworks
Monosaccharides - analysis
Nanostructures - chemistry
Organometallic Compounds - chemistry
Peptides
Peptides - analysis
Proteins - analysis
Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Starch - analysis
Surface Properties
Surface-assisted laser desorption–ionization mass spectrometry
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Summary:A 3D metal-organic framework (MOF) nanomaterial as matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and tandem mass spectrometry (MS/MS) was developed for the analysis of complex biomolecules. Unlike other nanoparticle matrices, this MOF nanomaterial does not need chemical modification prior to use. An exceptional signal reproducibility as well as very low background interferences in analyzing mono-/di-saccharides, peptides and complex starch digests demonstrate its high potential for biomolecule assays, especially for small molecules. [Display omitted] •This is the first report on the SALDI-MS analysis of polar carbohydrates using MOF as matrix.•3D MOF for SALDI-MS matrix was developed for biomolecule analysis without chemical modification.•Good signal reproducibility and low background interferences were achieved.•MOF for SALDI-MS can be used for quantitative and qualitative analysis of polar carbohydrate molecules.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2015.07.029