Pathogenic Association and Management of Botryodiplodia theobromae in Guava Orchards at Sheikhupura District, Pakistan

A survey was carried out in five tehsils of District Sheikhupura for the assessment of guava decline. Maximum disease prevalence (100%) and disease incidence (36%) was recorded in Tehsil Sharaqpur. Samples of plant roots, shoots and soil were randomly collected for the isolation and identification o...

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Bibliographic Details
Published in:International journal of agriculture and biology 2015-04, Vol.17 (2)
Main Authors: Safdar, Asma, Sajid Aleem Khan, Muhammad Arslan Safdar
Format: Article
Language:English
Subjects:
Aspergillus flavus
Botryodiplodia theobromae
Curvularia lunata
Fusarium oxysporum
Helminthosporium
Lasiodiplodia theobromae
Phytophthora parasitica
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Summary:A survey was carried out in five tehsils of District Sheikhupura for the assessment of guava decline. Maximum disease prevalence (100%) and disease incidence (36%) was recorded in Tehsil Sharaqpur. Samples of plant roots, shoots and soil were randomly collected for the isolation and identification of pathogens. Mean Colonization percentage of the Botryodiplodia theobromae Patouillard [Lasiodiplodia theobromae (Patouillard) Griffon and Maublanc] from 1987 tissues of 326 samples was counted to be (48.84%) maximum, followed by Fusarium oxysporum f.sp. psidii (44.10%), Phytophthora parasitica (38.10%), F. solani (35.10%) Helminthosporium spp. (15.20%) and Curvularia lunata (11.20%). Aspergillus flavus and A. niger were also isolated from the samples but mostly from twigs. Isolated fungi were multiplied and purified on PDA. Most dominating isolated fungus (B. theobromae) was evaluated for pathogenicity. Management of the B. theobromae was done under in vitro and in vivo conditions. In vitro efficacy of seven fungicides viz. carbendazim, thiophanate-methyl, alliette, acrobat MZ 75/667WP, dithan M45 80% WP, mancozeb 80% WP and metalaxyl plus mancozeb 72% WP was evaluated against B. theobromae by poisoned food technique at different doses viz. recommended (R), 0.75R, 0.50 and 0.25R. All the employed doses of the test fungicides significantly reduced the biomass of the test fungal specie but recommended dose rate reduced more significantly. In field experiment, carbendazim was found to be more effective than other fungicides checked, in reducing the fungal infection in guava trees, suppressing the dieback and wilting with significant enhancement in vegetative growth of plants. Copyright 2015 Friends Science Publishers
ISSN:1560-8530
1814-9596