Evolution of porcine reproductive and respiratory syndrome virus GP5 and GP3 genes under swIFN-β immune pressure and interferon regulatory factor-3 activation suppressed by GP5

• Published in: Research in veterinary science 2015-08, Vol.101, p.175-179
• Main Authors: Zhixuan, Xiong, Xiaoyun, Niu, Yabing, Song, Danping, Su, Fei, Wang, Rui-ai, Chen, Dongsheng, He
• Format: Article
• Language: English
• Subjects: Animals; Blotting, Western; Cell Culture Techniques; Cell Line; Cercopithecus aethiops; DNA-Binding Proteins - genetics; Electrophoresis, Polyacrylamide Gel; Evolution, Molecular; GP5 (glycoprotein-5); Interferon Regulatory Factor-3 - metabolism; Interferon-beta - metabolism; IRF3 (interferon regulatory factor-3); Mutations; Phosphorylation; Poly I-C; Porcine respiratory and reproductive syndrome virus; Porcine respiratory and reproductive syndrome virus - genetics; PRRSV (porcine reproductive and respiratory syndrome virus); swIFN-β (swine interferon-β); Swine - virology; Viral Envelope Proteins - genetics
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2015.05.007

In this study, a highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) strain, PRRSV GD07 was continuously propagated in MARC-145 cell cultures primed with swIFN-β for 50 passages to develop the PRRSV GDβfn strains. And a control strain PRRSV GDfn was passaged without swIFN-β. The sequencing analysis indicated that under swIFN-β immune pressure, molecular variation of PRRSV GP5 was accelerated in gene (NS/S>2.50), and the acceleration of GP3 was not significant (NS/S