Quinone-Rich Poly(dopamine) Magnetic Nanoparticles for Biosensor Applications

• Published in: Chemphyschem 2014-12, Vol.15 (17), p.3742-3752
• Main Authors: Martín, Miriam, González Orive, Alejandro, Lorenzo-Luis, Pablo, Hernández Creus, Alberto, González-Mora, José Luis, Salazar, Pedro
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; biosensors; Chemistry; Colloidal state and disperse state; Dopamine; Exact sciences and technology; General and physical chemistry; Glucose - analysis; Glucose - metabolism; glucose oxidase; Glucose Oxidase - metabolism; Indoles - chemistry; Indoles - metabolism; magnetic properties; Magnetite Nanoparticles - chemistry; Microscopy; Nanoparticles; natural products; Physical and chemical studies. Granulometry. Electrokinetic phenomena; Polymers - chemistry; Polymers - metabolism; Quinones - chemistry; Quinones - metabolism; Spectrum analysis
• ISSN: 1439-4235; 1439-7641
• DOI: 10.1002/cphc.201402417

Novel core‐shell quinone‐rich poly(dopamine)–magnetic nanoparticles (MNPs) were prepared by using an in situ polymerization method. Catechol groups were oxidized to quinone by using a thermal treatment. MNPs were characterized by using X‐ray diffraction, X‐ray photoelectron spectroscopy, atomic force microscopy, magnetic force microscopy, UV/Vis, Fourier‐transform infrared spectroscopy, and electrochemical techniques. The hybrid nanomaterial showed an average core diameter of 17 nm and a polymer‐film thickness of 2 nm. The core‐shell nanoparticles showed high reactivity and were used as solid supports for the covalent immobilization of glucose oxidase (Gox) through Schiff base formation and Michael addition. The amount of Gox immobilized onto the nanoparticle surface was almost twice that of the nonoxidized film. The resulting biofunctionalized MNPs were used to construct an amperometric biosensor for glucose. The enzyme biosensor has a sensitivity of 8.7 mA M−1 cm−2, a low limit of detection (0.02 mM), and high stability for 45 days. Finally, the biosensor was used to determine glucose in blood samples and was checked against a commercial glucometer. Biosensors get magnetic: The synthesis and characterization of core‐shell Fe3O4@poly(dopamine) (pDA150)/glucose oxidase (Gox) nanoparticles and the construction of a Fe3O4@pDA/Gox‐modified screen‐printed carbon electrode (SPCE) for the detection of glucose in biological samples are discussed. PB=Prussian Blue, PW=Prussian White.