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Absence of the mec A Gene in Methicillin Resistant Staphylococcus aureus Isolated from Different Clinical Specimens in Shendi City, Sudan

Absolute dependence on mec A gene as the defining standard in determining the resistance of S. aureus to methicillin became the subject of distrust by many researchers. The present study aimed to determine the frequency of mec A gene in methicillin resistant S. aureus (MRSA) isolates using polymeras...

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Published in:BioMed research international 2015, Vol.2015, p.1-5
Main Authors: Elhassan, Mogahid M., Ozbak, Hani A., Hemeg, Hassan A., Elmekki, Miskelyemen A., Ahmed, Leila M.
Format: Article
Language:English
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Summary:Absolute dependence on mec A gene as the defining standard in determining the resistance of S. aureus to methicillin became the subject of distrust by many researchers. The present study aimed to determine the frequency of mec A gene in methicillin resistant S. aureus (MRSA) isolates using polymerase chain reaction and to correlate its presence to conventional method. In this regard, two hundred S. aureus isolates were collected from patients with different diseases attending different hospitals in Shandi City, Sudan. Phenotypic Kirby-Bauer method confirmed the existence of methicillin resistant S. aureus in 61.5% of the subjected isolates with MICs ranging from 4  μ g/mL to 256  μ g/mL when using E -test. However, when amplifying a 310 bp fragment of the mec A gene by PCR, twelve out of the 123 MRSA isolates (9.8%) were mec A negative, whereas all the 77 methicillin sensitive S. aureus (MSSA) were mec A negative. In conclusion, this study drew attention to the credibility of the mec A gene and its usefulness in the detection of all MRSA strains without referring to the traditional methods. Hence, it is highly recommended to consider alternative mechanisms for β -lactam resistance that may compete with mec A gene in the emergence of MRSA phenomenon in the community.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/895860