Simple sequence repeat (SSR) markers in the ectomycorrhizal fungus Laccaria bicolor for environmental monitoring of introduced strains and molecular ecology applications

Simple sequence repeat (SSR) markers were developed from SSR-enriched genome libraries for the ectomycorrhizal basidiomycete Laccaria bicolor. Seven markers were single-locus and amplified unambiguously in L. bicolor. The seven SSR markers were further characterized using an array of 15 L. bicolor s...

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Bibliographic Details
Published in:Mycological research 2006, Vol.110 (1), p.51-59
Main Authors: Jany, Jean-Luc, Bousquet, Jean, Gagné, André, Khasa, Damase P.
Format: Article
Language:English
Subjects:
alleles
Basidiomycetes
Basidiomycota
Biomarkers
DNA Primers
DNA, Fungal - analysis
DNA, Fungal - isolation & purification
Ecology
ectomycorrhizae
Environmental Monitoring
gene segregation
Genetic Linkage
Laccaria bicolor
linkage (genetics)
microsatellite repeats
Model species
molecular sequence data
Mycorrhizae - genetics
mycorrhizal fungi
Mycorrhizas
nucleotide sequences
Plant Roots - microbiology
Population biology
Repetitive Sequences, Nucleic Acid - genetics
Russulales
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Summary:Simple sequence repeat (SSR) markers were developed from SSR-enriched genome libraries for the ectomycorrhizal basidiomycete Laccaria bicolor. Seven markers were single-locus and amplified unambiguously in L. bicolor. The seven SSR markers were further characterized using an array of 15 L. bicolor strains representative of diverse origins worldwide. The observed number of alleles per locus varied from 5–9 and the values of observed heterozygosity from 0.167 to 0.667. The seven SSR loci could be amplified from DNA extracted from root tips of L. bicolor inoculated pine seedlings. All the L. bicolor ectomycorrhizas analysed exhibited the same SSR multi-locus profile as that detected for the UAMH8232 inoculant strain. The set of markers described represents a potent tool for the monitoring of introduced strains of L. bicolor and for molecular ecology applications.
ISSN:0953-7562
1469-8102
DOI:10.1016/j.mycres.2005.09.011