Psoriatic T cells reduce epidermal turnover time and affect cell proliferation contributed from differential gene expression

Psoriasis is mediated primarily by T cells, which reduce epidermal turnover time and affect keratinocyte proliferation. We aimed to identify differentially expressed genes (DEG) in T cells from normal, five pairs of monozygotic twins concordant or discordant for psoriasis, to determine whether these...

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Bibliographic Details
Published in:Journal of dermatology 2015-09, Vol.42 (9), p.874-880
Main Authors: Li, Junqin, Li, Xinhua, Hou, Ruixia, Liu, Ruifeng, Zhao, Xincheng, Dong, Feng, Wang, Chunfang, Yin, Guohua, Zhang, Kaiming
Format: Article
Language:English
Subjects:
Antigens, Bacterial
Biomarkers - metabolism
Case-Control Studies
Cell growth
Cell Proliferation
differentially expressed genes
epidermal turnover time
Epidermis - physiopathology
Gene expression
Gene Expression Profiling
Humans
keratinocyte
Keratinocytes - physiology
Ki-67 Antigen - metabolism
Lymphocytes
Proto-Oncogene Proteins c-bcl-2 - metabolism
Proto-Oncogene Proteins c-myc - metabolism
Psoriasis
Psoriasis - immunology
Psoriasis - metabolism
Psoriasis - physiopathology
T cells
T-Lymphocytes - metabolism
Tumor Suppressor Protein p53 - metabolism
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Summary:Psoriasis is mediated primarily by T cells, which reduce epidermal turnover time and affect keratinocyte proliferation. We aimed to identify differentially expressed genes (DEG) in T cells from normal, five pairs of monozygotic twins concordant or discordant for psoriasis, to determine whether these DEG may account for the influence to epidermal turnover time and keratinocyte proliferation. The impact of T cells on keratinocyte proliferation and epidermal turnover time were investigated separately by immunohistochemistry and cultured with 3H‐TdR. mRNA expression patterns were investigated by RNA sequencing and verified by real‐time reverse transcription polymerase chain reaction. After co‐culture with psoriatic T cells, the expression of Ki‐67, c‐Myc and p53 increased, while expression of Bcl‐2 and epidermal turnover time decreased. There were 14 DEG which were found to participate in the regulation of cell proliferation or differentiation. Psoriatic T cells exhibited the ability to decrease epidermal turnover time and affect keratinocyte proliferation because of the differential expression of PPIL1, HSPH1, SENP3, NUP54, FABP5, PLEKHG3, SLC9A9 and CHCHD4.
ISSN:0385-2407
1346-8138
DOI:10.1111/1346-8138.12961