Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins

Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics of testis tissue was a feasible approach to identify more MPs because testis tissues have higher gene expression levels. Here we combined proteomics and transcriptomics to su...

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Bibliographic Details
Published in:Journal of proteome research 2015-09, Vol.14 (9), p.3583-3594
Main Authors: Zhang, Yao, Li, Qidan, Wu, Feilin, Zhou, Ruo, Qi, Yingzi, Su, Na, Chen, Lingsheng, Xu, Shaohang, Jiang, Tao, Zhang, Chengpu, Cheng, Gang, Chen, Xinguo, Kong, Degang, Wang, Yujia, Zhang, Tao, Zi, Jin, Wei, Wei, Gao, Yuan, Zhen, Bei, Xiong, Zhi, Wu, Songfeng, Yang, Pengyuan, Wang, Quanhui, Wen, Bo, He, Fuchu, Xu, Ping, Liu, Siqi
Format: Article
Language:English
Subjects:
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Genomics
Humans
Male
Proteins - isolation & purification
Proteins - metabolism
Proteomics
Sequence Analysis, RNA
Tandem Mass Spectrometry
Testis - metabolism
Transcriptome
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Summary:Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics of testis tissue was a feasible approach to identify more MPs because testis tissues have higher gene expression levels. Here we combined proteomics and transcriptomics to survey gene expression in human testis tissues from three post-mortem individuals. Proteins were extracted and separated with glycine- and tricine-SDS-PAGE. A total of 9597 protein groups were identified; of these, 166 protein groups were listed as MPs, including 138 groups (83.1%) with transcriptional evidence. A total of 2948 proteins are designated as MPs, and 5.6% of these were identified in this study. The high incidence of MPs in testis tissue indicates that this is a rich resource for MPs. Functional category analysis revealed that the biological processes that testis MPs are mainly involved in are sexual reproduction and spermatogenesis. Some of the MPs are potentially involved in tumorgenesis in other tissues. Therefore, this proteogenomics analysis of individual testis tissues provides convincing evidence of the discovery of MPs. All mass spectrometry data from this study have been deposited in the ProteomeXchange (data set identifier PXD002179).
ISSN:1535-3893
1535-3907
DOI:10.1021/acs.jproteome.5b00435