A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry

Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities – confocal microscopy, fluorescence lifetime...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2015-07, Vol.51 (52), p.10510-10513
Main Authors: Kaur, Amandeep, Haghighatbin, Mohammad A, Hogan, Conor F, New, Elizabeth J
Format: Article
Language:English
Subjects:
Confocal
Flow Cytometry
Fluorescence
Fluorescence Resonance Energy Transfer
Fretting
HeLa Cells
Humans
Luminescent Proteins - chemical synthesis
Luminescent Proteins - chemistry
Microscopy
Microscopy, Confocal
Microscopy, Fluorescence
Monitoring
Monitors
Oxidation-Reduction
Oxidative Stress
Stresses
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Summary:Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities – confocal microscopy, fluorescence lifetime imaging and flow cytometry.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc03394b