Quantitative determination of dopamine in single rat pheochromocytoma cells by microchip electrophoresis with only one high-voltage power supply

We developed a method for the direct identification of dopamine in single cultured rat pheochromocytoma cells by capillary electrophoresis using an end‐channel carbon fiber nanoelectrode amperometric detector. The operation mode was designed to achieve single‐cell injection and lysis in microfluidic...

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Bibliographic Details
Published in:Journal of separation science 2015-07, Vol.38 (13), p.2357-2362
Main Authors: Sha, Cuicui, Fan, Yuejuan, Cheng, Jieke, Cheng, Han
Format: Article
Language:English
Subjects:
Adrenal Gland Neoplasms - metabolism
Adrenal Gland Neoplasms - pathology
Animals
Capillarity
Capillary electrophoresis
Carbon fiber nanoelectrodes
Density
Dopamine
Dopamine - metabolism
Electrical measurements
Electrophoresis
Electrophoresis, Microchip - methods
Liquids
Microchip electrophoresis
PC12 Cells
Pheochromocytoma - metabolism
Pheochromocytoma - pathology
Pheochromocytoma cells
Power supplies
Power supply
Rats
Reproducibility of Results
Semiconductors
Separation
Single-Cell Analysis
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Summary:We developed a method for the direct identification of dopamine in single cultured rat pheochromocytoma cells by capillary electrophoresis using an end‐channel carbon fiber nanoelectrode amperometric detector. The operation mode was designed to achieve single‐cell injection and lysis in microfluidic chip electrophoresis with only one high‐voltage power supply. The separation and detection conditions were optimized. Four catecholamines were baseline‐separated and determined with this system, and the cell density and liquid height of the reservoirs were accommodated for single cell loading, docking and analysis. The microchip capillary electrophoresis system was successfully applied to determine dopamine in single cultured rat pheochromocytoma cells.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201500009