Loading…
Biomimetic DNA nanoballs for oligonucleotide delivery
Abstract Here, we designed biomimetic DNA nanoballs for delivery of multiple antisense oligonucleotides (ASOs). DNA templates with ASOs-complementary sequences were amplified by rolling circle amplification (RCA). RCA products were loaded with two types of ASOs by hybridization, condensed using aden...
Saved in:
| Published in: | Biomaterials 2015-09, Vol.62, p.155-163 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c501t-3b9184b150a9d21f0ee4c2cb82c69255d3c1b9bce66eb411bef3211aab4942f83 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c501t-3b9184b150a9d21f0ee4c2cb82c69255d3c1b9bce66eb411bef3211aab4942f83 |
| container_end_page | 163 |
| container_issue | |
| container_start_page | 155 |
| container_title | Biomaterials |
| container_volume | 62 |
| creator | Kim, Mi-Gyeong Park, Joo Yeon Shim, Gayong Choi, Han-Gon Oh, Yu-Kyoung |
| description | Abstract Here, we designed biomimetic DNA nanoballs for delivery of multiple antisense oligonucleotides (ASOs). DNA templates with ASOs-complementary sequences were amplified by rolling circle amplification (RCA). RCA products were loaded with two types of ASOs by hybridization, condensed using adenovirus-derived Mu peptide, and coated with hyaluronic acid (HA) for delivery into CD44-overexpressing tumor cells. HA-coated, Mu peptide-condensed, dual ASO-loaded DNA nanoballs (HMA nanoballs) showed considerable cellular entry of Cy5-incorporated RCA product DNA and fluorescent ASOs, whereas Mu peptide-condensed, dual ASO-loaded DNA nanoballs (MA nanoballs) revealed limited uptake. Dual ASOs, Dz13 and OGX-427, delivered by HMA nanoballs could reduce the levels of protein targets and exert anticancer effects. Enhanced tumor distribution was observed for fluorescent HMA nanoballs than the corresponding MA nanoballs. Upon intravenous co-administration with doxorubicin, HMA nanoballs exerted the greatest anti-tumor effects among the groups. These results suggest HMA nanoballs as a nanoplatform for sequence-specific delivery of multiple ASOs and other functional oligonucleotides. |
| doi_str_mv | 10.1016/j.biomaterials.2015.04.037 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1709764098</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0142961215004123</els_id><sourcerecordid>1689623871</sourcerecordid><originalsourceid>FETCH-LOGICAL-c501t-3b9184b150a9d21f0ee4c2cb82c69255d3c1b9bce66eb411bef3211aab4942f83</originalsourceid><addsrcrecordid>eNqNkkFv1DAQhS0EokvhL6CIE5eEGcd2Yg5IpaUFqYIDcLZsZ4K8JHGxk0r770nYghAXehqN9L33pHnD2AuECgHVq33lQhztTCnYIVccUFYgKqibB2yHbdOWUoN8yHaAgpdaIT9hT3Lew7qD4I_ZCVcgVcPVjsm3q1UYaQ6-uPh4Vkx2is4OQy76mIo4hG9xWvxAcQ4dFR0N4ZbS4Sl71K_R9OxunrKvl---nL8vrz9dfTg_uy69BJzL2mlshUMJVncceyASnnvXcq80l7KrPTrtPClFTiA66muOaK0TWvC-rU_Zy6PvTYo_FsqzGUP2NAx2orhkgw3oRgnQ90JRaJR1839UtVrxum1wRV8fUZ9izol6c5PCaNPBIJitDLM3f5dhtjIMCAO_cp7f5SxupO6P9Pf1V-DiCNB6w9tAyWQfaPLUhUR-Nl0M98t584-NH8IUvB2-04HyPi5p2jRoMjdgPm9vsX3F2gsI5HX9E_3btS8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1689623871</pqid></control><display><type>article</type><title>Biomimetic DNA nanoballs for oligonucleotide delivery</title><source>Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list)</source><creator>Kim, Mi-Gyeong ; Park, Joo Yeon ; Shim, Gayong ; Choi, Han-Gon ; Oh, Yu-Kyoung</creator><creatorcontrib>Kim, Mi-Gyeong ; Park, Joo Yeon ; Shim, Gayong ; Choi, Han-Gon ; Oh, Yu-Kyoung</creatorcontrib><description>Abstract Here, we designed biomimetic DNA nanoballs for delivery of multiple antisense oligonucleotides (ASOs). DNA templates with ASOs-complementary sequences were amplified by rolling circle amplification (RCA). RCA products were loaded with two types of ASOs by hybridization, condensed using adenovirus-derived Mu peptide, and coated with hyaluronic acid (HA) for delivery into CD44-overexpressing tumor cells. HA-coated, Mu peptide-condensed, dual ASO-loaded DNA nanoballs (HMA nanoballs) showed considerable cellular entry of Cy5-incorporated RCA product DNA and fluorescent ASOs, whereas Mu peptide-condensed, dual ASO-loaded DNA nanoballs (MA nanoballs) revealed limited uptake. Dual ASOs, Dz13 and OGX-427, delivered by HMA nanoballs could reduce the levels of protein targets and exert anticancer effects. Enhanced tumor distribution was observed for fluorescent HMA nanoballs than the corresponding MA nanoballs. Upon intravenous co-administration with doxorubicin, HMA nanoballs exerted the greatest anti-tumor effects among the groups. These results suggest HMA nanoballs as a nanoplatform for sequence-specific delivery of multiple ASOs and other functional oligonucleotides.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2015.04.037</identifier><identifier>PMID: 26056726</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Amplification ; Antisense oligonucleotides ; Biomimetic condensation ; Biomimetic Materials - administration & dosage ; Biomimetic Materials - chemical synthesis ; Cell Line, Tumor ; Cell Survival - drug effects ; Cell Survival - genetics ; Cellular ; Dentistry ; Deoxyribonucleic acid ; Diffusion ; DNA nanoballs ; DNA, Antisense - administration & dosage ; DNA, Antisense - genetics ; Genetic Therapy - methods ; Humans ; Nanocapsules - administration & dosage ; Nanocapsules - chemistry ; Nanocapsules - ultrastructure ; Nanospheres - administration & dosage ; Nanospheres - chemistry ; Nanospheres - ultrastructure ; Nanostructure ; Neoplasms, Experimental - genetics ; Neoplasms, Experimental - therapy ; Oligonucleotides ; Particle Size ; Peptides ; Rolling circle amplification ; Sequence-specific loading ; Surgical implants ; Transfection - methods ; Treatment Outcome ; Tumors</subject><ispartof>Biomaterials, 2015-09, Vol.62, p.155-163</ispartof><rights>Elsevier Ltd</rights><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-3b9184b150a9d21f0ee4c2cb82c69255d3c1b9bce66eb411bef3211aab4942f83</citedby><cites>FETCH-LOGICAL-c501t-3b9184b150a9d21f0ee4c2cb82c69255d3c1b9bce66eb411bef3211aab4942f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26056726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Mi-Gyeong</creatorcontrib><creatorcontrib>Park, Joo Yeon</creatorcontrib><creatorcontrib>Shim, Gayong</creatorcontrib><creatorcontrib>Choi, Han-Gon</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><title>Biomimetic DNA nanoballs for oligonucleotide delivery</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Here, we designed biomimetic DNA nanoballs for delivery of multiple antisense oligonucleotides (ASOs). DNA templates with ASOs-complementary sequences were amplified by rolling circle amplification (RCA). RCA products were loaded with two types of ASOs by hybridization, condensed using adenovirus-derived Mu peptide, and coated with hyaluronic acid (HA) for delivery into CD44-overexpressing tumor cells. HA-coated, Mu peptide-condensed, dual ASO-loaded DNA nanoballs (HMA nanoballs) showed considerable cellular entry of Cy5-incorporated RCA product DNA and fluorescent ASOs, whereas Mu peptide-condensed, dual ASO-loaded DNA nanoballs (MA nanoballs) revealed limited uptake. Dual ASOs, Dz13 and OGX-427, delivered by HMA nanoballs could reduce the levels of protein targets and exert anticancer effects. Enhanced tumor distribution was observed for fluorescent HMA nanoballs than the corresponding MA nanoballs. Upon intravenous co-administration with doxorubicin, HMA nanoballs exerted the greatest anti-tumor effects among the groups. These results suggest HMA nanoballs as a nanoplatform for sequence-specific delivery of multiple ASOs and other functional oligonucleotides.</description><subject>Advanced Basic Science</subject><subject>Amplification</subject><subject>Antisense oligonucleotides</subject><subject>Biomimetic condensation</subject><subject>Biomimetic Materials - administration & dosage</subject><subject>Biomimetic Materials - chemical synthesis</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - genetics</subject><subject>Cellular</subject><subject>Dentistry</subject><subject>Deoxyribonucleic acid</subject><subject>Diffusion</subject><subject>DNA nanoballs</subject><subject>DNA, Antisense - administration & dosage</subject><subject>DNA, Antisense - genetics</subject><subject>Genetic Therapy - methods</subject><subject>Humans</subject><subject>Nanocapsules - administration & dosage</subject><subject>Nanocapsules - chemistry</subject><subject>Nanocapsules - ultrastructure</subject><subject>Nanospheres - administration & dosage</subject><subject>Nanospheres - chemistry</subject><subject>Nanospheres - ultrastructure</subject><subject>Nanostructure</subject><subject>Neoplasms, Experimental - genetics</subject><subject>Neoplasms, Experimental - therapy</subject><subject>Oligonucleotides</subject><subject>Particle Size</subject><subject>Peptides</subject><subject>Rolling circle amplification</subject><subject>Sequence-specific loading</subject><subject>Surgical implants</subject><subject>Transfection - methods</subject><subject>Treatment Outcome</subject><subject>Tumors</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkkFv1DAQhS0EokvhL6CIE5eEGcd2Yg5IpaUFqYIDcLZsZ4K8JHGxk0r770nYghAXehqN9L33pHnD2AuECgHVq33lQhztTCnYIVccUFYgKqibB2yHbdOWUoN8yHaAgpdaIT9hT3Lew7qD4I_ZCVcgVcPVjsm3q1UYaQ6-uPh4Vkx2is4OQy76mIo4hG9xWvxAcQ4dFR0N4ZbS4Sl71K_R9OxunrKvl---nL8vrz9dfTg_uy69BJzL2mlshUMJVncceyASnnvXcq80l7KrPTrtPClFTiA66muOaK0TWvC-rU_Zy6PvTYo_FsqzGUP2NAx2orhkgw3oRgnQ90JRaJR1839UtVrxum1wRV8fUZ9izol6c5PCaNPBIJitDLM3f5dhtjIMCAO_cp7f5SxupO6P9Pf1V-DiCNB6w9tAyWQfaPLUhUR-Nl0M98t584-NH8IUvB2-04HyPi5p2jRoMjdgPm9vsX3F2gsI5HX9E_3btS8</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Kim, Mi-Gyeong</creator><creator>Park, Joo Yeon</creator><creator>Shim, Gayong</creator><creator>Choi, Han-Gon</creator><creator>Oh, Yu-Kyoung</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150901</creationdate><title>Biomimetic DNA nanoballs for oligonucleotide delivery</title><author>Kim, Mi-Gyeong ; Park, Joo Yeon ; Shim, Gayong ; Choi, Han-Gon ; Oh, Yu-Kyoung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-3b9184b150a9d21f0ee4c2cb82c69255d3c1b9bce66eb411bef3211aab4942f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Advanced Basic Science</topic><topic>Amplification</topic><topic>Antisense oligonucleotides</topic><topic>Biomimetic condensation</topic><topic>Biomimetic Materials - administration & dosage</topic><topic>Biomimetic Materials - chemical synthesis</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - genetics</topic><topic>Cellular</topic><topic>Dentistry</topic><topic>Deoxyribonucleic acid</topic><topic>Diffusion</topic><topic>DNA nanoballs</topic><topic>DNA, Antisense - administration & dosage</topic><topic>DNA, Antisense - genetics</topic><topic>Genetic Therapy - methods</topic><topic>Humans</topic><topic>Nanocapsules - administration & dosage</topic><topic>Nanocapsules - chemistry</topic><topic>Nanocapsules - ultrastructure</topic><topic>Nanospheres - administration & dosage</topic><topic>Nanospheres - chemistry</topic><topic>Nanospheres - ultrastructure</topic><topic>Nanostructure</topic><topic>Neoplasms, Experimental - genetics</topic><topic>Neoplasms, Experimental - therapy</topic><topic>Oligonucleotides</topic><topic>Particle Size</topic><topic>Peptides</topic><topic>Rolling circle amplification</topic><topic>Sequence-specific loading</topic><topic>Surgical implants</topic><topic>Transfection - methods</topic><topic>Treatment Outcome</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Mi-Gyeong</creatorcontrib><creatorcontrib>Park, Joo Yeon</creatorcontrib><creatorcontrib>Shim, Gayong</creatorcontrib><creatorcontrib>Choi, Han-Gon</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Mi-Gyeong</au><au>Park, Joo Yeon</au><au>Shim, Gayong</au><au>Choi, Han-Gon</au><au>Oh, Yu-Kyoung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biomimetic DNA nanoballs for oligonucleotide delivery</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>62</volume><spage>155</spage><epage>163</epage><pages>155-163</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Here, we designed biomimetic DNA nanoballs for delivery of multiple antisense oligonucleotides (ASOs). DNA templates with ASOs-complementary sequences were amplified by rolling circle amplification (RCA). RCA products were loaded with two types of ASOs by hybridization, condensed using adenovirus-derived Mu peptide, and coated with hyaluronic acid (HA) for delivery into CD44-overexpressing tumor cells. HA-coated, Mu peptide-condensed, dual ASO-loaded DNA nanoballs (HMA nanoballs) showed considerable cellular entry of Cy5-incorporated RCA product DNA and fluorescent ASOs, whereas Mu peptide-condensed, dual ASO-loaded DNA nanoballs (MA nanoballs) revealed limited uptake. Dual ASOs, Dz13 and OGX-427, delivered by HMA nanoballs could reduce the levels of protein targets and exert anticancer effects. Enhanced tumor distribution was observed for fluorescent HMA nanoballs than the corresponding MA nanoballs. Upon intravenous co-administration with doxorubicin, HMA nanoballs exerted the greatest anti-tumor effects among the groups. These results suggest HMA nanoballs as a nanoplatform for sequence-specific delivery of multiple ASOs and other functional oligonucleotides.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>26056726</pmid><doi>10.1016/j.biomaterials.2015.04.037</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 2015-09, Vol.62, p.155-163 |
| issn | 0142-9612 1878-5905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1709764098 |
| source | Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list) |
| subjects | Advanced Basic Science Amplification Antisense oligonucleotides Biomimetic condensation Biomimetic Materials - administration & dosage Biomimetic Materials - chemical synthesis Cell Line, Tumor Cell Survival - drug effects Cell Survival - genetics Cellular Dentistry Deoxyribonucleic acid Diffusion DNA nanoballs DNA, Antisense - administration & dosage DNA, Antisense - genetics Genetic Therapy - methods Humans Nanocapsules - administration & dosage Nanocapsules - chemistry Nanocapsules - ultrastructure Nanospheres - administration & dosage Nanospheres - chemistry Nanospheres - ultrastructure Nanostructure Neoplasms, Experimental - genetics Neoplasms, Experimental - therapy Oligonucleotides Particle Size Peptides Rolling circle amplification Sequence-specific loading Surgical implants Transfection - methods Treatment Outcome Tumors |
| title | Biomimetic DNA nanoballs for oligonucleotide delivery |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-23T17%3A52%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biomimetic%20DNA%20nanoballs%20for%20oligonucleotide%20delivery&rft.jtitle=Biomaterials&rft.au=Kim,%20Mi-Gyeong&rft.date=2015-09-01&rft.volume=62&rft.spage=155&rft.epage=163&rft.pages=155-163&rft.issn=0142-9612&rft.eissn=1878-5905&rft_id=info:doi/10.1016/j.biomaterials.2015.04.037&rft_dat=%3Cproquest_cross%3E1689623871%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c501t-3b9184b150a9d21f0ee4c2cb82c69255d3c1b9bce66eb411bef3211aab4942f83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1689623871&rft_id=info:pmid/26056726&rfr_iscdi=true |