Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids

Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy. Hence, it is important to know the exact composition of the degr...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2015-06, Vol.87 (12), p.6103-6111
Main Authors: Gensberger, Sabrina, Knabner, Carina, Waibel, Reiner, Huppert, Jochen, Pischetsrieder, Monika
Format: Article
Language:English
Subjects:
Arrays
Biocompatibility
Biocompatible Materials - analysis
Biodegradation
Chromatography
Chromatography, High Pressure Liquid
Degradation
Dialysis Solutions - analysis
Fluid dynamics
Fluid flow
Fluids
Glucans - analysis
Hemodialysis
Mass spectrometry
Molecular Structure
Osmosis
Peritoneal Dialysis
Sterilization
Tandem Mass Spectrometry
Trapping
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Summary:Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy. Hence, it is important to know the exact composition of the degradation products to improve biocompatibility of PD fluids. Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC–DAD–MS/MS). For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids. Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo­[d]­imidazol-2-yl)­furan-2-yl)­methanol. Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids. Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis­(1H-benzo­[d]­imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.5b00665