Dopamine Electroanalysis Using Electrochemical Biosensors Prepared by a Sinusoidal Voltages Method

A biocomposite material of tyrosinase and poly(3,4‐ethylenedioxythiophene) was electrodeposited onto gold (Au) disk microelectrode arrays and Au interdigitated microband electrode arrays (IDEs) by using a combination of sinusoidal voltages and microgravimetric method to construct amperometric biosen...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2015-07, Vol.27 (7), p.1649-1659
Main Authors: Lupu, Stelian, Lete, Cecilia, Javier del Campo, Francisco
Format: Article
Language:English
Subjects:
Amperometric biosensor
Arrays
Biosensors
Dopamine
Electric potential
Electrolytic analysis
Enzymes
Microelectrode arrays
Polymerization
Sinusoidal voltages
Tyrosinase
Voltage
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Summary:A biocomposite material of tyrosinase and poly(3,4‐ethylenedioxythiophene) was electrodeposited onto gold (Au) disk microelectrode arrays and Au interdigitated microband electrode arrays (IDEs) by using a combination of sinusoidal voltages and microgravimetric method to construct amperometric biosensors for dopamine (DA). The main advantage of this new strategy consists in a reliable enzyme immobilization and in additional information related to the electropolymerization process. The determination of DA was successfully achieved in pharmaceutical formulations. The best analytical performances, in terms of the lowest limit of detection of 6×10−7 M DA and highest recovery and stability, were obtained for IDEs based biosensor.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201400680