Highly efficient extraction and lipase-catalyzed transesterification of triglycerides from Chlorella sp. KR-1 for production of biodiesel

•Lipid of Chlorella sp. KR-1 was extracted using dimethyl carbonate (DMC) and methanol.•DMC was also used as acyl acceptor and reaction solvent.•FAMEs with a yield of 293mg/g biomass were obtained using Novozyme 435 in DMC.•Novozyme 435 was re-used for ten times with activity loss below 10% of initi...

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Bibliographic Details
Published in:Bioresource technology 2013-11, Vol.147, p.240-245
Main Authors: Lee, Ok Kyung, Kim, Young Hyun, Na, Jeong-Geol, Oh, You-Kwan, Lee, Eun Yeol
Format: Article
Language:English
Subjects:
Biocatalysis
Biofuel production
Biofuels
Biological and medical sciences
Biotechnology
Bulk molding compounds
Chlorella
Chlorella - metabolism
Chlorella sp. KR-1
Chromatography, Gas
Conversion
Dimethyl carbonate
Energy
Esterification
Extraction
Fatty acid methyl esters
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Lipase - metabolism
Lipids
Methyl alcohol
Microalgae
Novozyme 435
Transesterification
Triglycerides
Triglycerides - isolation & purification
Triglycerides - metabolism
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Summary:•Lipid of Chlorella sp. KR-1 was extracted using dimethyl carbonate (DMC) and methanol.•DMC was also used as acyl acceptor and reaction solvent.•FAMEs with a yield of 293mg/g biomass were obtained using Novozyme 435 in DMC.•Novozyme 435 was re-used for ten times with activity loss below 10% of initial value. We developed a method for the highly efficient lipid extraction and lipase-catalyzed transesterification of triglyceride from Chlorella sp. KR-1 using dimethyl carbonate (DMC). Almost all of the total lipids, approximately 38.9% (w/w) of microalgae dry weight, were extracted from the dried microalgae biomass using a DMC and methanol mixture (7:3 (v/v)). The extracted triglycerides were transesterified into fatty acid methyl esters (FAMEs) using Novozyme 435 as the biocatalyst in DMC. Herein, DMC was used as the reaction medium and acyl acceptor. The reaction conditions were optimized and the FAMEs yield was 293.82mg FAMEs/g biomass in 6h of reaction time at 60°C in the presence of 0.2% (v/v) water. Novozyme 435 was reused more than ten times while maintaining relative FAMEs conversion that was greater than 90% of the initial FAMEs conversion.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2013.08.037