Electrochemical DNA biosensor for human papillomavirus 16 detection in real samples

• Published in: Analytica chimica acta 2013-12, Vol.804, p.258-263
• Main Authors: Campos-Ferreira, Danielly S., Nascimento, Gustavo A., Souza, Elaine V.M., Souto-Maior, Maria A., Arruda, Mariana S., Zanforlin, Deborah M.L., Ekert, Marek H.F., Bruneska, Danyelly, Lima-Filho, José L.
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; Biosensors; Deoxyribonucleic acid; Electrochemical DNA biosensor; Electrochemical Techniques - methods; Electrodes; Genes, Viral; Gold; Human; Human papillomavirus 16; Human papillomavirus 16 - genetics; Human papillomavirus 16 - isolation & purification; Humans; Limit of Detection; Mathematical analysis; Methylene blue; Methylene Blue - chemistry; Screen-printed electrode; Voltammetry
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2013.10.038

•Electrochemical biosensor was used to detecting human papillomavirus (HPV) in real samples from endocervical swabs.•The detection limit was of 18.13nM.•No hybridization with non-complementary sequence showed that the method is selective.•It can be an excellent approach to detect human papillomavirus (HPV) in real samples. An electrochemical DNA biosensor for human papillomavirus (HPV) 16 detection has been developed. For this proposed biosensor, l-cysteine was first electrodeposited on the gold electrode surface to form l-cysteine film (CYSFILM). Subsequently, HPV16-specific probe was immobilized on the electrode surface with CYSFILM. Electrochemistry measurement was studied by differential pulse voltammetry method (DPV). The measurement was based on the reduction signals of methylene blue (MB) before and after hybridization either between probe and synthetic target or extracted DNA from clinical samples. The effect of probe concentration was analyzed and the best results were seen at 1000nM. The hybridization detection presented high sensitivity and broad linear response to the synthetic-target concentration comprised between 18.75nM and 250nM as well as to a detection limit of 18.13nM. The performance of this biosensor was also investigated by checking probe-modified electrode hybridization with extracted DNA from samples. The results showed that the biosensor was successfully developed and exhibited high sensitivity and satisfactory selectivity to HPV16. These results allow for the possibility of developing a new portable detection system for HPVs and for providing help in making an effective diagnosis in the early stages of infection.