Characterization of flocculating agent from the self-flocculating microalga Scenedesmus obliquus AS-6-1 for efficient biomass harvest

In the present work, the extracellular biopolymers from the self-flocculating microalga Scenedesmus obliquus AS-6-1 were studied. It was revealed that the self-flocculation of the microalgal cells was mediated by cell wall-associated polysaccharides with a molecular weight of 127.9kDa. Sugar composi...

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Bibliographic Details
Published in:Bioresource technology 2013-10, Vol.145, p.285-289
Main Authors: Guo, Suo-Lian, Zhao, Xin-Qing, Wan, Chun, Huang, Zih-You, Yang, Yu-Liang, Asraful Alam, Md, Ho, Shih-Hsin, Bai, Feng-Wu, Chang, Jo-Shu
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomass
Biopolymers - analysis
Cell flocculation
Cell Wall - chemistry
Chlorella vulgaris
Flocculating
Flocculating agent
Flocculation - drug effects
Fructose
Fundamental and applied biological sciences. Psychology
Glucose
Hydrogen-Ion Concentration
Mannose
Microalgae harvesting
Molecular weight
Monomers
Polysaccharide
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Reagents
Scenedesmus - chemistry
Scenedesmus obliquus
Taiwan
Temperature
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Summary:In the present work, the extracellular biopolymers from the self-flocculating microalga Scenedesmus obliquus AS-6-1 were studied. It was revealed that the self-flocculation of the microalgal cells was mediated by cell wall-associated polysaccharides with a molecular weight of 127.9kDa. Sugar compositions analysis indicated that the monomers consist of glucose, mannose, galatose, rhamnose and fructose with the molar ratio of 8:5:3:2:1. Addition of 0.6mg/L purified flocculating agent resulted in the fast flocculation of freely suspended cells of S. obliquus and Chlorella vulgaris. The flocculating activity is stable between pH 6 and 8 and at 20–60°C.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2013.01.120