Study on the adsorption of DNA on Fe3O4 nanoparticles and on ionic liquid-modified Fe3O4 nanoparticles

We have investigated the adsorption of herring sperm DNA on Fe 3 O 4 magnetic nanoparticles (NPs) before and after modification with the ionic liquid 1-hexyl-3-methylimidazolium bromide. Experiments were performed in a batch mode, and the effects of DNA concentration, pH of the sample solution, ioni...

Full description

Saved in:
Bibliographic Details
Published in:Mikrochimica acta (1966) 2014, Vol.181 (1-2), p.45-53
Main Authors: Ghaemi, Maryam, Absalan, Ghodratollah
Format: Article
Language:English
Subjects:
Adsorption
Analytical Chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Contact
Deoxyribonucleic acid
EDTA
Endothermic reactions
Microengineering
Nanochemistry
Nanoparticles
Nanotechnology
Original Paper
Solvents
Surface chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have investigated the adsorption of herring sperm DNA on Fe 3 O 4 magnetic nanoparticles (NPs) before and after modification with the ionic liquid 1-hexyl-3-methylimidazolium bromide. Experiments were performed in a batch mode, and the effects of DNA concentration, pH of the sample solution, ionic strength, temperature, and contact time between reagents were optimized. An evaluation of the adsorption isotherm revealed that the Langmuir model better fits the equilibrium data than the Freundlich model. The maximum adsorption capacities of the unmodified and modified NPs, respectively, were found to be 11.8 and 19.8 mg DNA per gram of adsorbent. The adsorption of DNA onto the modified NPs was endothermic, while it was exothermic in the case of the unmodified NPs. The DNA can be desorbed from the modified surfaces of the NPs by using EDTA as the eluent. The NPs were able to adsorb about 90 ± 1.5 % of DNA after being recycled for three times. The method is simple, fast, robust, and does not require organic solvents or sophisticated equipment. Figure Fe 3 O 4 nanoparticles as well as 1-hexyl-3-methylimidazolium bromidecoated Fe 3 O 4 nanoparticles were prepared and used for adsorption of DNA. The mean size and the surface morphology of both nanoparticles were characterized by TEM, DLS, XRD, FTIR and TGA techniques. The correlation coefficient of the Langmuir model suggests a better fit for the experimental equilibrium adsorption data.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-013-1040-5