A method for flow cytometric analysis of Candida albicans DNA

A method was developed by which to simply and easily study the DNA synthetic cycle of Candida albicans. This method employs a standard flow cytometer and the fluorescent DNA binding stain, 4,6-diamidino-2-phenylindole (DAPI). Flow-cytometric analysis by this method resulted in sharp peaks of intense...

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Bibliographic Details
Published in:Journal of microbiological methods 1998-08, Vol.33 (3), p.191-196
Main Authors: Stöver, A.G, Witek-Janusek, L, Mathews, H.L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Candida albicans
Cell cycle analysis
Flow cytometry
Fundamental and applied biological sciences. Psychology
Microbiology
Mycological methods and techniques used in mycology
Mycology
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Summary:A method was developed by which to simply and easily study the DNA synthetic cycle of Candida albicans. This method employs a standard flow cytometer and the fluorescent DNA binding stain, 4,6-diamidino-2-phenylindole (DAPI). Flow-cytometric analysis by this method resulted in sharp peaks of intense DNA staining. This method is a modification of a widely used protocol for cell cycle analysis in Saccharomyces cerevisiae that was found unsuitable for use with C. albicans. The S. cerevisiae procedure employed propidium iodide which stains C. albicans in a non-specific manner. The use of DAPI resulted in precise C. albicans nuclear staining and eliminated non-specific cellular staining.
ISSN:0167-7012
1872-8359
DOI:10.1016/S0167-7012(98)00029-3