Rapid discrimination of psychrotolerant and mesophilic strains of the Bacillus cereus group by PCR targeting of 16S rDNA

The paper describes a novel PCR assay for discriminating psychrotolerant and mesophilic strains of the Bacillus cereus group by targeting of 16S rDNA signatures. Application of the assay circumvents long-term growth tests at low temperature currently used to detect psychrotolerant strains. PCR was p...

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Bibliographic Details
Published in:Journal of microbiological methods 1998-11, Vol.34 (2), p.99-106
Main Authors: von Stetten, Felix, Francis, Kevin P., Lechner, Sabine, Neuhaus, Klaus, Scherer, Siegfried
Format: Article
Language:English
Subjects:
16S rDNA signature
ADN
BACILLUS CEREUS
Bacillus mycoides
Bacillus thuringiensis
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
DNA
Fundamental and applied biological sciences. Psychology
MESOPHILE MICROORGANISMS
Microbiology
MICROORGANISME MESOPHILE
MICROORGANISME PSYCHROPHILE
MICROORGANISMOS MESOFILOS
MICROORGANISMOS PSICROFILOS
PCR
PSYCHROPHILIC MICROORGANISMS
Psychrotolerant
TEMPERATURA
TEMPERATURE
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Summary:The paper describes a novel PCR assay for discriminating psychrotolerant and mesophilic strains of the Bacillus cereus group by targeting of 16S rDNA signatures. Application of the assay circumvents long-term growth tests at low temperature currently used to detect psychrotolerant strains. PCR was performed with pure cultures. A 100% correlation of PCR and growth data at 7°C was obtained for the 194 B. cereus group strains tested. Potential applications of the assay for the dairy industry and agriculture are suggested.
ISSN:0167-7012
1872-8359
DOI:10.1016/S0167-7012(98)00077-3