Three new continuous fish cell lines of SPH, SPS and RSBF derived from sea perch ( Lateolabrax japonicus) and red sea bream ( Pagrosomus major)

• Published in: Aquaculture 1998-11, Vol.169 (1), p.143-151
• Main Authors: Tong, Shang-Liang, Miao, Hong-Zhi, Li, Hong
• Format: Article
• Language: English
• Subjects: ACUICULTURA; Animal cells; AQUACULTURE; BESUGO; Biological and medical sciences; Biotechnology; BIRNAVIRUS; CELL CULTURE; Cell line; CULTIVO DE CELULAS; CULTURE DE CELLULE; DORADE; Establishment of new cell lines, improvement of cultural methods, mass cultures; Eukaryotic cell cultures; Fish; Fundamental and applied biological sciences. Psychology; LATEOLABRAX; Lateolabrax japonicus; Methods. Procedures. Technologies; Pagrosomas major; Pagrosomus major; Red sea bream; SEA BREAM; Sea perch
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/S0044-8486(98)00329-9

The continuous fish cell lines SPH and SPS were derived from heart and spleen tissues of sea perch Lateolabrax japonicus in 1993, and the cell line RSBF from fin tissue of red sea bream Pagrosomus major in 1994. They were subcultured for 106, 102 and 96 times respectively in the medium Eagle's MEM supplemented with 10% FBS. The growth temperature range was 15 to 30°C for all of the cell lines with the optimum growth at 25°C for SPH and SPS and at 30°C for RSBF. The distribution of chromosome number was 30 to 96 for SPH at passage 70, 36 to 86 for SPS at passage 65 and 10 to 62 for RSBF at passage 62. Nevertheless, the modal number of the chromosomes of all cell lines was 48. All of the cell lines were susceptible to fish birnavirus IPNV, though the titers were relatively low.